Growth of succinate consumer Dialister hominis is supported by Bacteroides thetaiotaomicron

Abstract

This study revealed an interaction between the gut commensal bacterium Bacteroides thetaiotaomicron JCM 5827^T and asaccharolytic bacterium Dialister hominis JCM 33369^T, which uses succinate instead of carbohydrates for growth. D. hominis usually forms extremely small colonies on Brucella blood agar plates. However, when co-cultured with B. thetaiotaomicron, D. hominis grew noticeably and formed larger colonies than those in the single culture, especially near B. thetaiotaomicron colonies. Although D. hominis barely grew in Gifu anaerobic medium broth, adding 1% succinate improved its growth. In the mixed culture, the succinate produced by B. thetaiotaomicron was mostly converted to propionate. This result was consistent with the single culture of D. hominis in the succinate-containing broth and our previous report on Phascolarctobacterium faecium, a succinate-utilizing gut bacterium. Our series of studies suggests that syntrophy within the human gut microbiota occurs via succinate.

Keywords: Bacteroides thetaiotaomicron; Dialister hominis; Succinate consumer; Succinate producer.