Protective Effect on Bone of Nacre Supplementation in Ovariectomized Rats

Dung Kim Nguyen; Norbert Laroche; Arnaud Vanden-Bossche; Marie-Thérèse Linossier; Mireille Thomas; Sylvie Peyroche; Myriam Normand; Yacine Bertache-Djenadi; Thierry Thomas; Hubert Marotte; Laurence Vico; Marie-Hélène Lafage-Proust; Marthe Rousseau

doi:10.1002/jbm4.10655

Protective Effect on Bone of Nacre Supplementation in Ovariectomized Rats

JBMR Plus. 2022 Jul 15;6(9):e10655. doi: 10.1002/jbm4.10655. eCollection 2022 Sep.

Authors

Dung Kim Nguyen¹, Norbert Laroche¹, Arnaud Vanden-Bossche¹, Marie-Thérèse Linossier¹, Mireille Thomas¹, Sylvie Peyroche¹, Myriam Normand¹, Yacine Bertache-Djenadi¹, Thierry Thomas^{1

2}, Hubert Marotte^{1

2}, Laurence Vico¹, Marie-Hélène Lafage-Proust^{1

2}, Marthe Rousseau^{1

3}

Affiliations

¹ U1059 SAINBIOSE, INSERM Jean Monnet University, University of Lyon, Mines Saint-Étienne Saint-Étienne France.
² Department of Rheumatology Hôpital Nord, CHU Saint-Etienne Saint-Etienne France.
³ University of Lyon, INSA-Lyon, CNRS, MATEIS (UMR 5510) Villeurbanne France.

Abstract

Nacre has emerged as a beneficial natural product for bone cells and tissues, but its effect was only studied by gavage in the ovariectomized mouse model. We sought to assess the antiosteoporotic effect of nacre through a nutritional supplementation in the ovariectomized rat model. Sixteen-week-old female Wistar rats were either Sham-operated or bilateral ovariectomized (OVX) and then fed with standard diet (Sham and OVX groups) or standard diet supplemented with either 0.25% CaCO₃ or nacre (OVX CaCO₃ and OVX Nacre group, respectively) for 28 days (n = 10/group). The bone microarchitecture was assessed at appendicular and axial bones by micro-computed tomography (μCT). Histomorphometric analysis was performed to determine cellular and dynamic bone parameters. Bone metabolism was also evaluated by biochemical markers and gene expression levels. Nacre-based diet prevented the OVX-induced bone loss better than that of the CaCO₃ supplement, given the significant changes in trabecular bone volume fraction (BV/TV) both at the femoral distal metaphysis (difference, 35%; p = 0.004) and at the second lumbar spine (difference, 11%; p = 0.01). Trabecular osteoclast surfaces (Oc.S/BS) were also 1.5-fold lower at the tibial proximal metaphysis in OVX Nacre group compared with OVX CaCO₃ group (p = 0.02)_. By principal component analysis (PCA), OVX Nacre group formed a cluster away from OVX group and with a trend closest to Sham group. These data were consistent with biological measurements demonstrating a positive profile related to nacre supplementation, which blunted an increase in serum CTX level and enhanced serum P1NP secretion 14 days post-OVX compared with CaCO₃ supplementation. Bmp2 mRNA expression in OVX Nacre group was +1.76-fold (p = 0.004) and +1.30-fold (p = 0.20) compared with OVX and OVX CaCO₃ groups, respectively. We conclude that supplementation with nacre could effectively limit bone loss induced by estrogen deficiency just after OVX in rats by modulating the negative imbalance of bone turnover. © 2022 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.

Keywords: BONE; NACRE; OSTEOPOROSIS; OVARIECTOMY; RATS.