DNA Visualization Using Fluorescent Proteins

Xuelin Jin; Y Tehee Kim; Kyubong Jo

doi:10.1007/978-1-0716-2667-2_11

DNA Visualization Using Fluorescent Proteins

Methods Mol Biol. 2023:2564:223-246. doi: 10.1007/978-1-0716-2667-2_11.

Authors

Xuelin Jin¹, Y Tehee Kim², Kyubong Jo³

Affiliations

¹ College of Agriculture, Yanbian University, Yanji, Jilin Province, China. 0000008832@ybu.edu.cn.
² Department of Chemistry and Program of Integrated Biotechnology, Sogang University, Seoul, Korea.
³ Department of Chemistry and Program of Integrated Biotechnology, Sogang University, Seoul, Korea. jokyubong@sogang.ac.kr.

PMID: 36107345
DOI: 10.1007/978-1-0716-2667-2_11

Abstract

DNA binding fluorescent proteins are a powerful tool for single-molecule visualization. In this chapter, we discuss a protocol for the synthesis of DNA binding fluorescent proteins and visualization of single DNA molecules. This chapter includes stepwise methods for molecular cloning, reversible staining, two-color staining, sequence-specific staining, and microscopic visualization of single DNA molecules in a microfluidic device. This content will be useful for DNA characterization using DNA binding fluorescent proteins and its visualization at the single-molecule level.

Keywords: Characteristics; DNA binding fluorescent protein; Microfluidic device; Sequence-specific staining; Single-molecule observation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA* / chemistry
DNA-Binding Proteins* / metabolism
Fluorescent Dyes
Microscopy, Fluorescence / methods
Staining and Labeling

Substances

DNA-Binding Proteins
Fluorescent Dyes
DNA