Angiogenesis in Free-Standing Two-Vasculature-Embedded Scaffold Extruded by Two-Core Laminar Flow Device

Chanh Trung Nguyen; Van Thuy Duong; Chang Ho Hwang; Kyo-In Koo

doi:10.18063/ijb.v8i3.557

Angiogenesis in Free-Standing Two-Vasculature-Embedded Scaffold Extruded by Two-Core Laminar Flow Device

Int J Bioprint. 2022 May 13;8(3):557. doi: 10.18063/ijb.v8i3.557. eCollection 2022.

Authors

Chanh Trung Nguyen¹, Van Thuy Duong¹, Chang Ho Hwang², Kyo-In Koo¹

Affiliations

¹ Major of Biomedical Engineering, Department of Electrical, Electronic and Computer Engineering, University of Ulsan, Ulsan, Republic of Korea.
² Department of Physical and Rehabilitation Medicine, Chungnam National University Sejong Hospital, College of Medicine, Chungnam National University, Sejong, Republic of Korea.

Abstract

Rapid construction of pre-vascular structure is highly desired for engineered thick tissue. However, angiogenesis in free-standing scaffold has been rarely reported because of limitation in growth factor (GF) supply into the scaffold. This study, for the 1^st time, investigated angiogenic sprouting in free-standing two-vasculature-embedded scaffold with three different culture conditions and additional GFs. A two-core laminar flow device continuously extruded one vascular channel with human umbilical vein endothelial cells (HUVECs) and a 3 mg/ml type-1 collagen, one hollow channel, and a shell layer with 2% w/v gelatin-alginate (70:30) composite. Under the GF flowing condition, angiogenic sprouting from the HUVEC vessel had started since day 1 and gradually grew toward the hollow channel on day 10. Due to the medium flowing, the HUVECs showed elongated spindle-like morphology homogeneously. Their viability has been over 80% up to day 10. This approach could apply to vascular investigation, and drug discovery further, not only to the engineered thick tissue.

Keywords: Angiogenesis; Culture condition; Free standing; Gelatin-alginate; Pre-vascularized tissue; Two-core vasculature.