Intestinal absorptivity-increasing effects of sodium N-[8-(2-hydroxybenzoyl)amino]-caprylate on food-derived bioactive peptide

Yongzhao Xu; William Kwame Amakye; Ganhong Xiao; Xuebo Liu; Jiaoyan Ren; Min Wang

doi:10.1016/j.foodchem.2022.134059

Intestinal absorptivity-increasing effects of sodium N-[8-(2-hydroxybenzoyl)amino]-caprylate on food-derived bioactive peptide

Food Chem. 2023 Feb 1:401:134059. doi: 10.1016/j.foodchem.2022.134059. Epub 2022 Sep 6.

Authors

Yongzhao Xu¹, William Kwame Amakye¹, Ganhong Xiao¹, Xuebo Liu², Jiaoyan Ren³, Min Wang⁴

Affiliations

¹ School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
² College of Food Science and Engineering, Northwest A&F University, Yangling, China.
³ School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; Research Institute for Food Nutrition and Human Health, Guangzhou, China. Electronic address: jyren@scut.edu.cn.
⁴ School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China. Electronic address: wangmin1990@scut.edu.cn.

PMID: 36095999
DOI: 10.1016/j.foodchem.2022.134059

Abstract

Delivering bioactive peptides orally is hampered by poor absorption across the gastrointestinal barrier. Using the walnut-derived peptide PW5, PPKNW, we explored whether coformulation of peptides with absorption enhancer sodium N-[8-(2-hydroxybenzoyl)aminocaprylate] (SNAC) could improve the intestinal absorption of orally-administered bioactive peptides. Herein, the application of SNAC enhanced the absorption efficiency of PW5 in a non-everted gut sac model. Particle size distribution (1 027.8 ± 6.74 nm) and zeta potential (-2.89 ± 0.07 mV) of the PW5-SNAC complex were significantly greater than that of individual PW5 and SNAC. Scanning electron microscopy revealed that SNAC application could aggravate the surface roughness and reduce the compact structure of PW5. It further showed that PW5 and SNAC binds through an endothermic process underpinned by hydrogen bond and van der Waals forces and that SNAC could bound primarily to the internal calyx of PW5. These findings are helpful for the effective delivery of bioactive peptides.

Keywords: Absorption enhancer; Bioactive peptide; Interaction; Intestinal absorptivity; PW5; SNAC.

MeSH terms

Caprylates*
Intestinal Absorption
Peptides / pharmacology
Sodium* / pharmacology

Substances

octanoic acid
Caprylates
Sodium
Peptides