Cytotoxicity of two self-adhesive resin cements and their interference in the phagocytic activity of murine macrophages

Danilo Couto da Silva; Leonardo Gomes Vaz; Warley Luciano Fonseca Tavares; Leda Quercia Vieira; Ricardo Reis de Oliveira; Antônio Paulino Ribeiro Sobrinho

doi:10.5395/rde.2022.47.e31

Cytotoxicity of two self-adhesive resin cements and their interference in the phagocytic activity of murine macrophages

Restor Dent Endod. 2022 Jul 14;47(3):e31. doi: 10.5395/rde.2022.47.e31. eCollection 2022 Aug.

Authors

Danilo Couto da Silva¹, Leonardo Gomes Vaz², Warley Luciano Fonseca Tavares¹, Leda Quercia Vieira², Ricardo Reis de Oliveira¹, Antônio Paulino Ribeiro Sobrinho¹

Affiliations

¹ Department of Restorative Dentistry, Faculty of Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
² Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.

Abstract

Objectives: This study aimed to evaluate in vitro the effects of the self-adhesive resin cements RelyX U200 (3M ESPE) and seT PP (SDI Limited) on murine macrophages and the interference of the photoactivation.

Materials and methods: Cell viability assays, cell adherence, yeast phagocytosis of Saccharomyces boulardii and production of reactive oxygen species (ROS) were performed in the presence of capillaries containing the respective self-adhesive cement when photoactivated or not.

Results: After long periods of contact, both types of cements, when not photoactivated, are more cytotoxic for macrophages. The seT PP cement when only chemically activated seems to interfere more negatively in the process of phagocytosis of yeasts S. boulardii. Both types of cements interfere in the cell adhesion process, independent of photoactivation. None of the types of cements tested was able to induce the production of ROS.

Conclusions: Our results highlight the great importance of the photoactivation of self-adhesive resin cements in the dental clinic, since RelyX U200, when photoactivated, presented the best results within the evaluated parameters.

Keywords: Cytotoxicity; Dental cement; Macrophages; Resin cement.