Transcriptomics provides insights into toxicological effects and molecular mechanisms associated with the exposure of Chinese mitten crab, Eriocheir sinensis, to dioxin

Abstract

Dioxins are stable, ubiquitous, persistent, and halogenated environmental pollutants that have recently garnered increasing attention. This study constructed a microcosmic system to simulate the real breeding conditions of the Chinese mitten crab (Eriocheir sinensis) to evaluate the impact of environmental dioxins on these aquaculture animals. Histological observation and detection of antioxidant enzyme activities revealed that dioxin exposure for different durations substantially damaged the hepatopancreas of Chinese mitten crabs, increasing the enzymatic activities of total superoxide dismutase (T-SOD) and catalase (CAT) but decreasing that of malondialdehyde (MDA). We also obtained the gene expression profiles of the hepatopancreas corresponding to different periods of dioxin exposure using RNA-seq technology. Compared with the control group, 2999 and 941 differentially expressed genes (DEGs) corresponding to different periods of dioxin exposure were identified in the hepatopancreas. Enrichment analysis indicated that some pathways, such as those governing carbohydrate metabolism, fatty acid metabolism, and immune disease, also responded to dioxin exposure. Subsequently, we selectively analyzed DEGs involved in oxidoreductase activity, carbohydrate metabolic processes, and other processes, identifying that increased expression of Hsp70, Ldh, and Trx1 and decreased expression of Lgbp, Bgal1, and Acsbg2 were potentially caused by sensitivity to environmental dioxin exposure. Therefore, we contend that, although crabs exposed to unfavorable environmental pollutants, such as dioxin, may adapt via antioxidant and immune response modulation. However, continued dioxin exposure would disrupt such homeostatic restorative capabilities. Thus, this study may provide new insights into the toxicological effects exerted by dioxin on aquatic organisms, such as E. sinensis, as well as the mechanisms underlying such toxicity.

Keywords: Dioxin; Eriocheir sinensis; Immune response; Transcriptome.