Biodegradation of polyethylene by Meyerozyma guilliermondii and Serratia marcescens isolated from the gut of waxworms (larvae of Plodia interpunctella)

Abstract

The widespread use of polyethylene (PE) causes a large amount of indigestible plastic waste. Waxworms (the larvae of Plodia interpunctella) can eat PE, but the degradation principle of PE under the action of intestinal microorganisms is still unclear, especially the insufficient research on key degradable PE strains. In this study, we fed waxworms with PE. Two strains with high PE degradation efficiency were isolated and purified, and the effects of single and microbial consortia on PE degradation were evaluated by water contact angle (WCA), FTIR, GC-MS, SEM and RT-qPCR. The results showed that Meyerozyma guilliermondii ZJC1 (MgZJC1) and Serratia marcescens ZJC2 (SmZJC2) could degrade PE. However, the degradation efficiency of the microbial consortium was higher, and the weight loss rate of PE was 15.87 %. In addition, the PE degradation products of MgZJC1 were C₉H₁₀O, C₂₀H₁₅NO, C₂₈H₄₄O₃ and C₁₆H₃₂O₂, and the PE degradation products of SmZJC2 were C₁₆H₁₈O, C₁₄H₁₈N₂O₇ and C₃₁H₄₈O₆. The PE degradation products of the microbial consortium were C₁₁H₂₄, C₁₉H₁₀O, C₁₅H₃₂, C₁₄H₃₀, C₁₆H₃₄, C₂₅H₅₂ and C₂₇H₅₆. RT-qPCR results showed that SmZJC2 promoted PE degradation by upregulating the expression of multiple genes, such as multicopper oxidase genes (PiSm-CueO). MgZJC1 responded to carbon deficiency by upregulating the expression of multiple genes, such as key enzyme genes in the tricarboxylic acid (TCA) cycle. This study can be used to develop an efficient microbial consortium for PE degradation and provide a basis for the reuse of PE waste. It can also provide a research basis for the joint degradation of PE by microbial consortia composed of bacteria and fungi.

Keywords: Biodegradation; Biofilms; Gene expression; Microbial consortium; Plastics.