Synthesis and Characterization of a new Alginate-Gelatine Aerogel for Tissue Engineering

Annu Int Conf IEEE Eng Med Biol Soc. 2022 Jul:2022:3915-3918. doi: 10.1109/EMBC48229.2022.9871508.

Abstract

Scaffolds have been used to stimulate cell migration, cell adhesion, and cell proliferation as extracellular matrix analogues. This study proposes a novel method for creating hybrid alginate-gelatine aerogel-based scaffold, which could be suitable for cell adhesion. To this end, alginate-gelatine at 4% was first used to make stable hydrogels, which were then frozen at -70°C and dried under a vacuum to produced aerogels. Aerogels are materials known for their extremely low density, which, by definition, should be lower than 0.5 g/cm3, In this study, a bulk density of 0.16 g/cm3 was reached, confirming that the created material fits within the definition of an aerogel. In addition, the material presented a sponge-like structure, high absorption properties, and high-porosity, with an average pore size of 193μm. These properties fit within the requirements for fibroblast cell infiltrate and survival, demonstrating that the proposed alginate-gelatine aerogels are suitable candidates for various applications such as tissue engineering and regenerative medicine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alginates / chemistry
  • Gelatin* / chemistry
  • Hydrogels
  • Tissue Engineering* / methods
  • Tissue Scaffolds / chemistry

Substances

  • Alginates
  • Hydrogels
  • Gelatin