In this contribution, the development and validation of a digital twin of a decentralized source of breathing gas used for mechanical ventilation is described. A hardware setup is built in analogy to a Simscape ® model with almost identical architecture and geometry. An experimental trial of volume controlled ventilation was conducted and the obtained data of resulting pressure and oxygen concentration was compared. Correlating measured and simulated data lead to coefficients of 0.83 and 0.74 for FiO2 in the dosage chamber and airway, respectively. Clinical relevance- This work showed that the process of mechanical ventilation can be simulated even to the extent of oxygen distribution for ex-vivo applications.