Prevention of mastitis in multiparous dairy cows with a previous history of mastitis by oral feeding with probiotic Bacillus subtilis

Megumi Urakawa; Tao Zhuang; Hidetoshi Sato; Satoru Takanashi; Kozue Yoshimura; Yuma Endo; Teppei Katsura; Tsuyoshi Umino; Koutaro Tanaka; Hitoshi Watanabe; Hiroko Kobayashi; Naokazu Takada; Tomoyuki Kozutsumi; Hiroaki Kumagai; Takafumi Asano; Kohko Sazawa; Nobuhisa Ashida; Guoqi Zhao; Michael T Rose; Haruki Kitazawa; Hitoshi Shirakawa; Kouichi Watanabe; Tomonori Nochi; Takehiko Nakamura; Hisashi Aso

doi:10.1111/asj.13764

Prevention of mastitis in multiparous dairy cows with a previous history of mastitis by oral feeding with probiotic Bacillus subtilis

Anim Sci J. 2022 Jan-Dec;93(1):e13764. doi: 10.1111/asj.13764.

Authors

Megumi Urakawa^{1

2

3}, Tao Zhuang^{1

2

3}, Hidetoshi Sato⁴, Satoru Takanashi^{1

3}, Kozue Yoshimura^{1

2

3}, Yuma Endo^{1

2

3}, Teppei Katsura^{1

2

3}, Tsuyoshi Umino^{1

3}, Koutaro Tanaka^{1

3}, Hitoshi Watanabe³, Hiroko Kobayashi⁴, Naokazu Takada⁴, Tomoyuki Kozutsumi⁴, Hiroaki Kumagai⁴, Takafumi Asano⁴, Kohko Sazawa⁴, Nobuhisa Ashida², Guoqi Zhao⁵, Michael T Rose⁶, Haruki Kitazawa^{1

2

7}, Hitoshi Shirakawa^{1

2

8}, Kouichi Watanabe^{1

2

3}, Tomonori Nochi^{1

2

3}, Takehiko Nakamura^{1

2}, Hisashi Aso^{1

2

3

9}

Affiliations

¹ International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
² Laboratory of Animal Health Science, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
³ Laboratory of Animal Functional Morphology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
⁴ Miyagi Prefectural Livestock Experiment Station, Osaki, Japan.
⁵ Institute of Animal Culture Collection and Application, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
⁶ Tasmanian Institute of Agriculture, University of Tasmania, Sandy Bay, Tasmania, Australia.
⁷ Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
⁸ Laboratory of Nutrition, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
⁹ The Cattle Museum, Maesawa, Oshu, Japan.

Abstract

Mastitis is a very common inflammatory disease of the mammary gland of dairy cows, resulting in a reduction of milk production and quality. Probiotics may serve as an alternative to antibiotics to prevent mastitis, and the use of probiotics in this way may lessen the risk of antibiotic resistant bacteria developing. We investigated the effect of oral feeding of probiotic Bacillus subtilis (BS) C-3102 strain on the onset of mastitis in dairy cows with a previous history of mastitis. BS feeding significantly decreased the incidence of mastitis, the average number of medication days and the average number of days when milk was discarded, and maintained the mean SCC in milk at a level substantially lower than the control group. BS feeding was associated with lower levels of cortisol and TBARS and increased the proportion of CD4⁺ T cells and CD11c⁺ CD172a^high dendritic cells in the blood by flow cytometry analysis. Parturition increased the migrating frequency of granulocytes toward a milk chemoattractant cyclophilin A in the control cows, however, this was reduced by BS feeding, possibly indicating a decreased sensitivity of peripheral granulocytes to cyclophilin A. These results reveal that B. subtilis C-3102 has potential as a probiotic and has preventative capacity against mastitis in dairy cows.

Keywords: Bacillus subtilis C-3102 strain; bovine mastitis; dairy cattle; probiotics; prophylactic effect.

MeSH terms

Animals
Anti-Bacterial Agents / therapeutic use
Bacillus subtilis
Cattle
Cattle Diseases*
Cyclophilin A
Female
Mastitis, Bovine* / prevention & control
Probiotics*

Substances

Anti-Bacterial Agents
Cyclophilin A

Abstract

MeSH terms

Substances

Grants and funding