In-vitro cytotoxicity of nickel oxide nanoparticles against L-6 cell-lines: MMP, MTT and ROS studies

Renu Bala; Bhawna Pareek; Ahmad Umar; Saroj Arora; Davinder Singh; Ashun Chaudhary; Abdulrab Ahmed M Alkhanjaf; Abdulrhman A Almadiy; Hassan Algadi; Raman Kumar; Vivek Sheel Jaswal; Sotirios Baskoutas

doi:10.1016/j.envres.2022.114257

In-vitro cytotoxicity of nickel oxide nanoparticles against L-6 cell-lines: MMP, MTT and ROS studies

Environ Res. 2022 Dec;215(Pt 1):114257. doi: 10.1016/j.envres.2022.114257. Epub 2022 Sep 6.

Authors

Affiliations

¹ Department of Chemistry, Maharishi Markandeshwar (Deemed to be University), Mullana, 133207, Ambala, India.
² Department of Chemistry, College of Science and Arts, and Promising Centre for Sensors and Electronics Devices (PCSED), Najran University, Najran, 11001, Saudi Arabia; Department of Materials Science and Engineering, The Ohio State University, Columbus, OH, 43210, USA. Electronic address: ahmadumar786@gmail.com.
³ Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, 143005, Punjab, India.
⁴ Department of Plant Science, Central University of Himachal Pradesh, Dharamshala, District Kangra, Himachal Pradesh, 176215, India.
⁵ Molecular Diagnostics, Clinical Laboratory Sciences Department, College of Applied Medical Sciences, Najran University, Najran, 11001, Saudi Arabia.
⁶ Department of Biology, College of Science and Arts, Najran University, Najran, 11001, Saudi Arabia.
⁷ Department of Electrical Engineering, College of Engineering, Najran University, Najran, 11001, Saudi Arabia.
⁸ Department of Chemistry, Maharishi Markandeshwar (Deemed to be University), Mullana, 133207, Ambala, India; Department of Biotechnology, MMEC, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala, 133207, Haryana, India. Electronic address: ramankumar4@gmail.com.
⁹ Department of Chemistry, Maharishi Markandeshwar (Deemed to be University), Mullana, 133207, Ambala, India; Department of Chemistry and Chemical Science, Central University of Himachal Pradesh, Dharmshala, District Kangra, Himachal Pradesh, 176215, India. Electronic address: chemsheel@gmail.com.
¹⁰ Department of Materials Science, University of Patras, 26500, Patras, Greece.

PMID: 36084676
DOI: 10.1016/j.envres.2022.114257

Abstract

In the present work we synthesize nickel oxide nanoparticles (NiO NPs) using Rhododendron arboretum (flower) (RNi), Tinospora cordifolia (stems) (GNi), Corylus jacquemontii (seeds) (CNi), and Nardostachys jatamansi (roots) (NNi) extracts by co-precipitation method. The synthesized NiO NPs were characterized in detail in terms of their morphological, crystalline nature, structural and antiproliferative activity against rat skeletal myoblast (L-6) cell lines. Morphological studies confirmed the formation of nanoparticles, while the structural and compositional characterization revealed the well-crystallinity and high purity of the synthesized nanoparticles. For biological applications and cytotoxicity examinations of the synthesized NPs, the rat skeletal myoblast (L-6) cell lines were subjected to study. By detailed cytotoxic investigations, it was observed that among the four kinds of NiO NPs prepared through different plant extracts, the Tinospora cordifolia (stems) showed strong antiproliferative activity against rat skeletal myoblast (L-6) cell lines and the calculated IC₅₀ was 1.671 mg/mL. The observed antiproliferative activity towards different NiO NPs were in the order of GNi > NNi > RNi > CNi. The present studies demonstrate that simply synthesized NiO can efficiently be used as antiproliferative agents.

Keywords: Biological properties; Green synthesis; MTT assays.; Nickel Oxide nanoparticles.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antineoplastic Agents*
Metal Nanoparticles* / chemistry
Metal Nanoparticles* / toxicity
Nanoparticles*
Nickel / metabolism
Nickel / toxicity
Plant Extracts / pharmacology
Rats
Reactive Oxygen Species / metabolism

Substances

Antineoplastic Agents
Plant Extracts
Reactive Oxygen Species
Nickel
nickel monoxide