Gold nanobipyramids (AuBPs) with narrow size distribution and high monodispersity have driven intensive attention because they display more advantageous plasmonic properties than gold nanorods (AuNRs). Applications of AuBPs based on tunable plasmonic properties and enhanced electromagnetic fields are being widely investigated in recent years. In this article, we focused on the preparation of well-defined AuBPs using the seed-mediated method, the plasmonic properties, and the exploration of AuBP-supported derivatives. The synergetic contributions of penta-twinned and appropriate growth environment could produce high-purity AuBPs. Systematic comparisons of plasmonic properties between AuBPs and AuNRs are illustrated. In addition, the well-defined AuBPs can be used as a template to synthesize multi-metallic nanostructures. The development of the epitaxial growth based on the AuBPs and corresponding applications are introduced. This study will provide a guide for the fabrication of composite nanostructures and advance their plasmonic applications.