A novel conductive nanocomposite-based biosensor for ultrasensitive detection of microRNA-21 in serum, using methylene blue as mediator

Mengtian Deng; Jialu Feng; Dan Tao; Hanhui Yan; Jingjing Ding; Nicole Jaffrezic-Renault; Zhenzhong Guo

doi:10.1016/j.bioelechem.2022.108256

A novel conductive nanocomposite-based biosensor for ultrasensitive detection of microRNA-21 in serum, using methylene blue as mediator

Bioelectrochemistry. 2022 Dec:148:108256. doi: 10.1016/j.bioelechem.2022.108256. Epub 2022 Sep 5.

Authors

Mengtian Deng¹, Jialu Feng², Dan Tao³, Hanhui Yan¹, Jingjing Ding⁴, Nicole Jaffrezic-Renault⁵, Zhenzhong Guo⁶

Affiliations

¹ Hubei Province Key Laboratory of Occupational Hazard identification and Control, Wuhan University of Science and Technology, Wuhan 430065, China; School of Public Health, Medical College, Wuhan University of Science and Technology, Wuhan 430065, China.
² Hubei Province Key Laboratory of Occupational Hazard identification and Control, Wuhan University of Science and Technology, Wuhan 430065, China.
³ School of Public Health, the key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, China.
⁴ School of Basic Medicine, Medical College, Wuhan University of Science and Technology, Wuhan 430065, China.
⁵ University of Lyon, Institute of Analytical Sciences, UMR-CNRS 5280, 5, La Doua Street, Villeurbanne 69100, France. Electronic address: nicole.jaffrezic@univ-lyon1.fr.
⁶ Hubei Province Key Laboratory of Occupational Hazard identification and Control, Wuhan University of Science and Technology, Wuhan 430065, China. Electronic address: zhongbujueqi@hotmail.com.

PMID: 36081272
DOI: 10.1016/j.bioelechem.2022.108256

Abstract

MicroRNA-21 (miRNA-21) is a common biomarker with high expression in breast tumors. Therefore, sensitive detection of miRNA-21 is of great significance for clinical breast tumor diagnosis. A TH/rGO/CMK-3/AuNPs nanocomposite is composed of thionine (TH), reduced graphene oxide (rGO), ordered mesoporous carbon (CMK-3), and gold nanoparticles (AuNPs), which help to increase the specific surface area of a glassy carbon electrode (GCE) and to amplify the DPV signal. Meanwhile, methylene blue (MB) was combined with the capture probe guanine and absorbed by the composite material to mediate the differential pulse voltammetry (DPV) of the obtained miRNA biosensor. The current response decreased with increasing miRNA-21 concentration under optimal conditions. The biosensor responds to miRNA-21 in the 0.1fM-1 pM concentration range, and the detection limit (LOD) was 0.046 fM. Moreover, human serum samples were effectively detected utilizing the miRNA-21 biosensor with satisfactory results.

Keywords: Biosensor; Breast cancer; Human serum; Methylene blue mediator; MicroRNA-21; TH/rGO/CMK-3/AuNPs.

MeSH terms

Biosensing Techniques* / methods
Carbon
Electrochemical Techniques / methods
Gold
Graphite*
Guanine
Humans
Limit of Detection
Metal Nanoparticles*
Methylene Blue
MicroRNAs*
Nanocomposites*

Substances

MIRN21 microRNA, human
MicroRNAs
graphene oxide
Guanine
Carbon
Gold
Graphite
Methylene Blue