Colorimetric Approach for Nucleic Acid Salmonella spp. Detection: A Systematic Review

Asma Nadia Ahmad Faris; Mohamad Ahmad Najib; Muhammad Najmi Mohd Nazri; Amir Syahir Amir Hamzah; Ismail Aziah; Nik Yusnoraini Yusof; Rohimah Mohamud; Irneza Ismail; Fatin Hamimi Mustafa

doi:10.3390/ijerph191710570

Colorimetric Approach for Nucleic Acid Salmonella spp. Detection: A Systematic Review

Int J Environ Res Public Health. 2022 Aug 25;19(17):10570. doi: 10.3390/ijerph191710570.

Authors

Asma Nadia Ahmad Faris¹, Mohamad Ahmad Najib¹, Muhammad Najmi Mohd Nazri¹, Amir Syahir Amir Hamzah², Ismail Aziah¹, Nik Yusnoraini Yusof¹, Rohimah Mohamud³, Irneza Ismail⁴, Fatin Hamimi Mustafa¹

Affiliations

¹ Institute for Research in Molecular Medicine (INFORMM), Health Campus, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia.
² Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia.
³ Department of Immunology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia.
⁴ Advanced Devices & System (ADS) Research Group, Department of Electrical & Electronic Engineering, Faculty of Engineering and Built Environment, Universiti Sains Islam Malaysia, Bandar Baru Nilai, Nilai 71800, Negeri Sembilan, Malaysia.

Abstract

Water- and food-related health issues have received a lot of attention recently because food-poisoning bacteria, in particular, are becoming serious threats to human health. Currently, techniques used to detect these bacteria are time-consuming and laborious. To overcome these challenges, the colorimetric strategy is attractive because it provides simple, rapid and accurate sensing for the detection of Salmonella spp. bacteria. The aim of this study is to review the progress regarding the colorimetric method of nucleic acid for Salmonella detection. A literature search was conducted using three databases (PubMed, Scopus and ScienceDirect). Of the 88 studies identified in our search, 15 were included for further analysis. Salmonella bacteria from different species, such as S. Typhimurium, S. Enteritidis, S. Typhi and S. Paratyphi A, were identified using the colorimetric method. The limit of detection (LoD) was evaluated in two types of concentrations, which were colony-forming unit (CFU) and CFU per mL. The majority of the studies used spiked samples (53%) rather than real samples (33%) to determine the LoDs. More research is needed to assess the sensitivity and specificity of colorimetric nucleic acid in bacterial detection, as well as its potential use in routine diagnosis.

Keywords: Salmonella; colorimetric; detection; nucleic acid; systematic review.

Publication types

Review
Systematic Review
Research Support, Non-U.S. Gov't

MeSH terms

Colorimetry*
Humans
Limit of Detection
Nucleic Acids*
Salmonella / genetics
Sensitivity and Specificity

Substances

Nucleic Acids

Grants and funding

This research was funded by the Fundamental Research Grant Scheme from the Ministry of Higher Education Malaysia (Grant number FRGS/1/2019/TK04/USM/03/1, Account No: 203.CIPPM.6171221) and Higher Institution Centre of Excellence (HICoE) Grant from the Ministry of Higher Education, Malaysia (311/CIPPM/4401005). The article processing charge was funded by the Universiti Sains Islam Malaysia (USIM).