Skin proteomic profiling of irradiation-induced fibrosis and its modulation by low molecular weight fucoidan via tight junction pathway

Pang-Hung Hsu; Yi-Hsuan Chen; Pin-I Huang; Pai-An Hwang

doi:10.1016/j.biopha.2022.113417

Skin proteomic profiling of irradiation-induced fibrosis and its modulation by low molecular weight fucoidan via tight junction pathway

Biomed Pharmacother. 2022 Sep:153:113417. doi: 10.1016/j.biopha.2022.113417. Epub 2022 Jul 20.

Authors

Pang-Hung Hsu¹, Yi-Hsuan Chen², Pin-I Huang³, Pai-An Hwang⁴

Affiliations

¹ Department of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan; Bachelor Degree Program in Marine Biotechnology, National Taiwan Ocean University, Keelung, Taiwan; Institute of Biochemistry and Molecular Biology, National Yang Ming Chiao Tung University, Taipei, Taiwan; Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, Taiwan.
² Department of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan.
³ Department of Oncology, Division of Radiation Oncology, Taipei Veterans General Hospital School of Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan.
⁴ Department of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan; Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, Taiwan. Electronic address: amperehwang@email.ntou.edu.tw.

PMID: 36076539
DOI: 10.1016/j.biopha.2022.113417

Abstract

Fucoidans, sulfated and fucosylated polysaccharides extracted from brown seaweed, were found to inhibit radiotherapy-induced cell damage and fibrosis through the TGF-β1 pathway. However, the comprehensive molecular response during irradiation-induced fibrosis and fucoidan-assisted recovery still remain unclear. Rat hind limbs were irradiated and smeared with low molecular weight fucoidan (LMF). Protein profiles were examined by a mass spectrometry-based proteomics analysis. Out of a total of 4625 proteins, 233 were found to be significantly up-regulated after irradiation and down-regulated after LMF treatment. Pathway and protein-protein interaction network analyses further indicated that four proteins including Actb, Ezr, Msn and Cdc42 were clustered into the tight junction and regulation of actin cytoskeleton pathways. These four proteins may serve as biomarkers for the detection of skin fibrosis induced by irradiation or TGF-β1, and for the recovery following LMF treatment.

Keywords: Fibrosis; Fucoidan; Protein interaction maps; Proteomics; Radiotherapy; Tight junctions.

MeSH terms

Animals
Antineoplastic Agents*
Fibrosis
Molecular Weight
Polysaccharides / pharmacology
Proteomics
Rats
Tight Junctions
Transforming Growth Factor beta1*

Substances

Antineoplastic Agents
Polysaccharides
Transforming Growth Factor beta1
fucoidan