Allophycocyanin A is a carbon dioxide receptor in the cyanobacterial phycobilisome

Abstract

Light harvesting is fundamental for production of ATP and reducing equivalents for CO₂ fixation during photosynthesis. However, electronic energy transfer (EET) through a photosystem can harm the photosynthetic apparatus when not balanced with CO₂. Here, we show that CO₂ binding to the light-harvesting complex modulates EET in photosynthetic cyanobacteria. More specifically, CO₂ binding to the allophycocyanin alpha subunit of the light-harvesting complex regulates EET and its fluorescence quantum yield in the cyanobacterium Synechocystis sp. PCC 6803. CO₂ binding decreases the inter-chromophore distance in the allophycocyanin trimer. The result is enhanced EET in vitro and in live cells. Our work identifies a direct target for CO₂ in the cyanobacterial light-harvesting apparatus and provides insights into photosynthesis regulation.