A successful case of assisted reproduction after contamination of the culture medium with Escherichia coli

Eur Rev Med Pharmacol Sci. 2022 Aug;26(16):5932-5938. doi: 10.26355/eurrev_202208_29533.

Abstract

Introduction: We report a case of successful pregnancy after in vitro fertilization culture (IVF) medium contaminated with Escherichia coli (E. coli).

Case report: To remove E. coli, oocytes were isolated in a PVP-ICSI medium and then in a culture medium supplemented with gentamicin. We selected two embryos that were vitrified and frozen. In the next natural cycle, we performed the embryo transfer of 2 embryos.

Results: Only one pregnancy was uncomplicated until term and was completed by the live birth of a healthy baby. The child's subsequent development until the date of publication of this article is normal.

Conclusions: In cases of E. coli contamination of the IVF culture medium, specific laboratory methods allow the procedure to be continued, and a healthy baby can be obtained.

Publication types

  • Case Reports

MeSH terms

  • Embryo Transfer* / methods
  • Escherichia coli*
  • Female
  • Fertilization in Vitro / methods
  • Humans
  • Live Birth
  • Oocytes
  • Pregnancy
  • Pregnancy Rate