A phage-based magnetic relaxation switching biosensor using bioorthogonal reaction signal amplification for Salmonella detection in foods

Chenxi Huang; Junpeng Zhao; Rongsheng Lu; Jia Wang; Sam R Nugen; Yiping Chen; Xiaohong Wang

doi:10.1016/j.foodchem.2022.134035

A phage-based magnetic relaxation switching biosensor using bioorthogonal reaction signal amplification for Salmonella detection in foods

Food Chem. 2023 Jan 30:400:134035. doi: 10.1016/j.foodchem.2022.134035. Epub 2022 Aug 28.

Authors

Chenxi Huang¹, Junpeng Zhao², Rongsheng Lu³, Jia Wang², Sam R Nugen⁴, Yiping Chen⁵, Xiaohong Wang⁶

Affiliations

¹ Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, Hubei, China; Department of Food Science, Cornell University, Ithaca, NY 14853, USA.
² Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
³ Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, Nanjing 211189, China.
⁴ Department of Food Science, Cornell University, Ithaca, NY 14853, USA.
⁵ Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, Hubei, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China. Electronic address: chenyiping@mail.hzau.edu.cn.
⁶ Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, Hubei, China. Electronic address: wxh@mail.hzau.edu.cn.

PMID: 36063677
DOI: 10.1016/j.foodchem.2022.134035

Abstract

Phages are uniquely suited for bacterial detection due to their low cost and ability to recognize live bacteria. Herein, our work establishes the proof-of-concept detection of Salmonella in orange juice based on a phage-mediated portable magnetic relaxation switching (MRS) biosensor. The limit of quantification (LOQ) could reach 5 CFU/mL (95 % confidence interval [CI]: 4-7, N = 4) with a linear range of 10²-10⁸ CFU/mL, which has improved 10-fold than that without bioorthogonal signal amplification. The recovery rate of the phage-based MRS biosensor was 95.0 % (95 % confidence interval [CI]: 89.0 %-100.9 %, N = 6). The specificity of the phage-based MRS biosensor was 100 % without false-positive results. In addition, this sensor was able to detect <10 CFU per 25 mL of Salmonella in orange juice with 4-h pre-enrichment. The result from the phage-based MRS biosensor is consistent with that from the standard plate count method. This sensor provides a reliable and ultrasensitive detection platform for pathogens.

Keywords: Bioorthogonal reaction; Magnetic relaxation switching (MRS); Phage; Salmonella detection; Signal amplification.

MeSH terms

Bacteriophages*
Biosensing Techniques* / methods
Magnetic Phenomena
Magnetics / methods
Salmonella