Effects of two alveolar recruitment maneuvers in an "open-lung" approach during laparoscopy in dogs

Caterina Di Bella; Caterina Vicenti; Joaquin Araos; Luca Lacitignola; Laura Fracassi; Marzia Stabile; Salvatore Grasso; Alberto Crovace; Francesco Staffieri

doi:10.3389/fvets.2022.904673

Effects of two alveolar recruitment maneuvers in an "open-lung" approach during laparoscopy in dogs

Front Vet Sci. 2022 Aug 18:9:904673. doi: 10.3389/fvets.2022.904673. eCollection 2022.

Authors

Caterina Di Bella¹, Caterina Vicenti², Joaquin Araos³, Luca Lacitignola², Laura Fracassi², Marzia Stabile², Salvatore Grasso⁴, Alberto Crovace², Francesco Staffieri²

Affiliations

¹ School of Bioscience and Veterinary Medicine, University of Camerino, Camerino, Italy.
² Section of Veterinary Clinics and Animal Production, Department of Emergency and Organ Transplantations, University of Bari, Bari, Italy.
³ Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States.
⁴ Section of Anesthesia and Intensive Care, Department of Emergency and Organ Transplantations, University of Bari, Bari, Italy.

Abstract

Objectives: The aim of this study was to compare the effects of a sustained inflation alveolar recruiting maneuver (ARM) followed by 5 cmH₂O of PEEP and a stepwise ARM, in dogs undergoing laparoscopic surgery.

Materials and methods: Twenty adult dogs were enrolled in this prospective randomized clinical study. Dogs were premedicated with methadone intramuscularly (IM); anesthesia was induced with propofol intravenously (IV) and maintained with inhaled isoflurane in pure oxygen. The baseline ventilatory setting (BVS) was as follows: tidal volume of 15 mL/kg, inspiratory pause of 25%, inspiratory to expiratory ratio of 1:2, and the respiratory rate to maintain the end-tidal carbon dioxide between 45 and 55 mmHg. 10 min after pneumoperitoneum, randomly, 10 dogs underwent sustained inflation ARM followed by 5 cmH₂O of PEEP (ARMi), while 10 dogs underwent a stepwise recruitment maneuver followed by the setting of the "best PEEP" (ARMc). Gas exchange, respiratory system mechanics, and hemodynamic were evaluated before the pneumoperitoneum induction (BASE), 10 min after the pneumoperitoneum (PP), 10 min after the recruitment (ARM), and 10 min after the pneumoperitoneum resolution (PostPP). Statistical analysis was performed with the ANOVA test (p < 0.05).

Results: Static compliance decreased in both groups at PP (ARMc = 1.35 ± 0.21; ARMi = 1.16 ± 0.26 mL/cmH₂O/kg) compared to BASE (ARMc = 1.78 ± 0.60; ARMi = 1.66 ± 0.66 mL/cmH₂O/kg) and at ARM (ARMc = 1.71 ± 0.41; ARMi = 1.44 ± 0.84 mL/cmH₂O/kg) and PostPP (ARMc = 1.75 ± 0.45; ARMi = 1.89 ± 0.59 mL/cmH₂O/kg), and it was higher compared to PP and similar to BASE. The PaO₂/FiO₂, in both groups, was higher at ARM (ARMc = 455.11 ± 85.90; ARMi = 505.40 ± 31.70) and PostPP (ARMc = 521.30 ± 66.20; ARMi = 450.90 ± 70.60) compared to PP (ARMc = 369.53 ± 49.31; ARMi = 394.32 ± 37.72).

Conclusion and clinical relevance: The two ARMs improve lung function in dogs undergoing laparoscopic surgery similarly. Application of PEEP at the end of the ARMs prolonged the effects of the open-lung strategy.

Keywords: alveolar recruitment; atelectasis; dog; laparoscopy; oxygenation.