Comparison of oxidative stress-mitochondria-mediated tenderization in two different bovine muscles during aging

Zhenjiang Ding; Qichao Wei; Chunmei Liu; Chunhui Zhang; Feng Huang

doi:10.1016/j.fochms.2022.100131

Comparison of oxidative stress-mitochondria-mediated tenderization in two different bovine muscles during aging

Food Chem (Oxf). 2022 Aug 22:5:100131. doi: 10.1016/j.fochms.2022.100131. eCollection 2022 Dec 30.

Authors

Zhenjiang Ding^{1

2}, Qichao Wei¹, Chunmei Liu¹, Chunhui Zhang¹, Feng Huang¹

Affiliations

¹ Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing 100193, China.
² Beijing Key Laboratory of the Innovative Development of Functional Staple and Nutritional Intervention for Chronic Diseases, China National Research Institute of Food and Fermentation Industries, Beijing 100015, China.

Abstract

The aim of this study was to investigate the differences in the effects of mitochondria-involved energy metabolism and caspases activation on postmortem tenderness in different muscle fiber types. Beef Longissimus thoracis (LT) and Psoas major (PM) muscles showed significant difference in mitochondrial function. Our data revealed that PM suffered from higher levels of reactive oxygen species (ROS) earlier than LT, causing faster mitochondrial swelling and rupture. Additionally, faster metabolism of ATP-related compounds and activation of caspase-9 appeared in PM, but the activity of caspase-3 in PM was lower than that in LT. Differences in myofibril fragmentation index (MFI) of LT and PM at different aging stages suggested that energy metabolism and caspases activities may play a role in tenderness at different aging stages. These results indicated that oxidative stress-mitochondria-mediated tenderization process could be muscle-specific.

Keywords: Caspases; Energy metabolism; Mitochondria; Oxidative stress; Tenderization.