Integrated driver mutations profile of chinese gastrointestinal-natural killer/T-cell lymphoma

Shanshan Li; Tingzhi Liu; Hailing Liu; Xiaohui Zhai; Taiyuan Cao; Hongen Yu; Wanjia Hong; Xiaoru Lin; Ming Li; Yan Huang; Jian Xiao

doi:10.3389/fonc.2022.976762

Integrated driver mutations profile of chinese gastrointestinal-natural killer/T-cell lymphoma

Front Oncol. 2022 Aug 18:12:976762. doi: 10.3389/fonc.2022.976762. eCollection 2022.

Authors

Shanshan Li^{1

2}, Tingzhi Liu^{2

3}, Hailing Liu^{2

4}, Xiaohui Zhai^{1

2}, Taiyuan Cao^{1

2}, Hongen Yu^{1

2}, Wanjia Hong^{1

2}, Xiaoru Lin^{1

2}, Ming Li^{1

2}, Yan Huang^{2

4}, Jian Xiao^{1

2}

Affiliations

¹ Department of Medical Oncology, The Sixth Affiliated Hospital of Sun-Yat Sen University, Guangzhou, China.
² Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
³ Department of Medical Hematology, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.
⁴ Department of Pathology, The Sixth Affiliated Hospital of Sun-Yat Sen University, Guangzhou, China.

Abstract

Background: One of the most common nasal external sites in extranodal Natural Killer/T-cell lymphoma (NKTCL) is in the gastrointestinal (GI) system. Despite this, reports on gastrointestinal-Natural Killer/T-cell lymphoma (GI-NKTCL) are very few. To obtain a better understanding of this manifestation of NKTCL, we conducted a retrospective study on GI-NKTCL to analyze its clinical features, genomic changes and immune infiltration.

Methods: We retrospectively collected patients diagnosed with GI-NKTCL in the Sixth Affiliated Hospital of Sun Yat-sen University from 2010 to 2020. From this cohort we obtained mutation data via whole exome sequencing.

Results: Genomic analysis from 15 patients with GI-NKTCL showed that the most common driving mutations were ARID1B(14%, 2/15), ERBB3(14%, 2/15), POT1(14%, 2/15), and TP53(14%, 2/15). In addition, we found the most common gene mutation in patients with GI-NKTCL to be RETSAT(29%, 4/15) and SNRNP70(21%, 3/15), and the most common hallmark pathway mutations to be G2M checkpoint pathway (10/15, 66.7%), E2F targets (8/15, 53.3%), estrogen response late (7/15, 46.7%), estrogen response early (7/15, 46.7%), apoptosis (7/15, 46.7%) and TNFA signaling via NFKB (7/15, 46.7%). In the ICIs-Miao cohort, SNRNP7-wild-type (WT) melanoma patients had significantly prolonged overall survival (OS) time compared with SNRNP7 mutant type (MT) melanoma patients. In the TCGA-UCEC cohort, the patients with RETSAT-MT or SNRNP7-MT had significantly increased expression of immune checkpoint molecules and upregulation of inflammatory immune cells.

Conclusions: In this study, we explored GI-NKTCL by means of genomic analysis, and identified the most common mutant genes (RETSAT and SNRNP70), pathway mutations (G2M checkpoint and E2F targets) in GI-NKTCL patients. Also, we explored the association between the common mutant genes and immune infiltration. Our aim is that our exploration of these genomic changes will aid in the discovery of new biomarkers and therapeutic targets for those with GI-NKTCL, and finally provide a theoretical basis for improving the treatment and prognosis of patients with GI-NKTCL.

Keywords: driver gene; gastrointestinal-natural killer/T-cell lymphoma (GI-NKTCL); genomic analysis; immune infiltration; mutation.