Chimpanzee and pig-tailed macaque iPSCs: Improved culture and generation of primate cross-species embryos

Morteza Roodgar; Fabian P Suchy; Lan H Nguyen; Vivek K Bajpai; Rahul Sinha; Jose G Vilches-Moure; Kevin Van Bortle; Joydeep Bhadury; Ahmed Metwally; Lihua Jiang; Ruiqi Jian; Rosaria Chiang; Angelos Oikonomopoulos; Joseph C Wu; Irving L Weissman; Joseph L Mankowski; Susan Holmes; Kyle M Loh; Hiromitsu Nakauchi; Catherine A VandeVoort; Michael P Snyder

doi:10.1016/j.celrep.2022.111264

Chimpanzee and pig-tailed macaque iPSCs: Improved culture and generation of primate cross-species embryos

Cell Rep. 2022 Aug 30;40(9):111264. doi: 10.1016/j.celrep.2022.111264.

Authors

Morteza Roodgar¹, Fabian P Suchy², Lan H Nguyen³, Vivek K Bajpai⁴, Rahul Sinha², Jose G Vilches-Moure⁵, Kevin Van Bortle⁶, Joydeep Bhadury⁷, Ahmed Metwally⁶, Lihua Jiang⁶, Ruiqi Jian⁶, Rosaria Chiang⁶, Angelos Oikonomopoulos⁸, Joseph C Wu⁸, Irving L Weissman², Joseph L Mankowski⁹, Susan Holmes¹⁰, Kyle M Loh¹¹, Hiromitsu Nakauchi¹², Catherine A VandeVoort¹³, Michael P Snyder¹⁴

Affiliations

¹ Department of Genetics, Stanford University, Stanford, CA 94305, USA; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA. Electronic address: mroodgar@stanford.edu.
² Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.
³ Institute for Computational and Mathematical Engineering, Stanford University, Stanford, CA 94305, USA.
⁴ Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Chemical and Systems Biology, Stanford University, Stanford, CA 94305, USA.
⁵ Department of Comparative Medicine, Stanford University, Stanford, CA 94305, USA.
⁶ Department of Genetics, Stanford University, Stanford, CA 94305, USA.
⁷ Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA; Institute of Biomedicine, Sahlgrenska University Hospital, University of Gothenburg, SE 413 45 Gothenburg, Sweden.
⁸ Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA.
⁹ Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
¹⁰ Department of Statistics, Stanford University, Stanford, CA 94305, USA.
¹¹ Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.
¹² Department of Genetics, Stanford University, Stanford, CA 94305, USA; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA. Electronic address: nakauchi@stanford.edu.
¹³ California National Primate Research Center and Department of Obstetrics and Gynecology, University of California, Davis, Davis, CA, USA. Electronic address: cavandevoort@ucdavis.edu.
¹⁴ Department of Genetics, Stanford University, Stanford, CA 94305, USA. Electronic address: mpsnyder@stanford.edu.

Abstract

As our closest living relatives, non-human primates uniquely enable explorations of human health, disease, development, and evolution. Considerable effort has thus been devoted to generating induced pluripotent stem cells (iPSCs) from multiple non-human primate species. Here, we establish improved culture methods for chimpanzee (Pan troglodytes) and pig-tailed macaque (Macaca nemestrina) iPSCs. Such iPSCs spontaneously differentiate in conventional culture conditions, but can be readily propagated by inhibiting endogenous WNT signaling. As a unique functional test of these iPSCs, we injected them into the pre-implantation embryos of another non-human species, rhesus macaques (Macaca mulatta). Ectopic expression of gene BCL2 enhances the survival and proliferation of chimpanzee and pig-tailed macaque iPSCs within the pre-implantation embryo, although the identity and long-term contribution of the transplanted cells warrants further investigation. In summary, we disclose transcriptomic and proteomic data, cell lines, and cell culture resources that may be broadly enabling for non-human primate iPSCs research.

Keywords: CP: Developmental biology; Nonhuman primates; blastocyst, BCL2; chimpanzee; cross-species embryos; iPSCs; interspecies chimera; pig-tailed macaque.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
Induced Pluripotent Stem Cells*
Macaca mulatta
Macaca nemestrina / genetics
Pan troglodytes*
Proteomics

Abstract

Publication types

MeSH terms

Grants and funding