The rapid development of single-cell technology has opened up a whole new perspective for identifying cell types in multicellular organisms and understanding the relationships between them. Distinguishing different cell types and subtypes can identify the components of different immune cells and different tumor clones in the tumor microenvironment, which is the basic work of tumor heterogeneity analysis and can help researchers understand the mechanism of tumor immune escape. Existing algorithms treat both cell types and subtypes as populations of cells with specific gene expression patterns, which is not conducive to accurate cell typing. For that, we proposed a cell similarity metric that unifies cell type recognition and subtype identification (UCRSI), with the assumption that selectively expressed genes represent differences in underlying cell type with on/off manner, while differences in expression level represent different cell subtype with more/less manner. Our method calculates these two kinds of differences separately, and then combines them using a consensus adjacency matrix, and finally cell typing is completed using spectral clustering algorithm. The results show that UCRSI can reconstruct expert annotation of single-cell RNA sequencing datasets more robustly than existing methods. And, UCRSI is useful for analyzing tumor heterogeneity and improving visualization of large-scale cell clustering.