Background: This study aims to compare serum HMGB-1, 3-nitrotyrosine (3-NT), TAS, TOS, and OSI levels in Wettype Age-Related Macular Degeneration (wAMD) patients and healthy controls to determine the correlation of these parameters with each other.
Methods: Thirty patients with Wet-type Age-Related Macular Degeneration (wAMD) and 27 healthy adults, as controls were enrolled in the study. We determined the TAS and TOS levels in serum samples of both groups using commercial kits on a microplate reader. Serum HMGB-1 and 3-NT levels were measured with the enzyme-linked immunosorbent assay method.
Results: HMGB-1 levels were significantly higher in the patient group (137.51 pg/mL, p=0.001), while there was no difference between the two groups in serum 3-NT levels (p=0.428). A statistically significant difference found in the levels of TOS and OSI (p=0.001 and p=0.045, respectively) between the patients and controls, however, no significant difference was observed between the groups in terms of TAS levels (p=0.228).
Conclusions: Oxidative stress and HMGB-1 levels were increased in wAMD patients and enhanced oxidative stress may be associated with increased tissue necrosis and inflammation. Thus administration of antioxidant treatment in addition to routine therapy should be considered in wAMD.
Uvod: Ova studija ima za cilj da uporedi serumske nivoe HMBG-1, 3-nitrotirozina (3-NT), TAS, TOS i OSI kod pacijenata sa vlažnom starosnom makularnom degeneracijom (vAMD) i zdravih kontrola kako bi se utvrdila korelacija ovih parametri međusobno.
Metode: Trideset pacijenata sa vlažnom starosnom makularnom degeneracijom (wAMD) i 27 zdravih odraslih osoba su uključene u studiju. Određivali smo nivoe TAS i TOS u uzorcima seruma obe grupe pomoću komercijalnih kompleta na čitaču mikroploča. Serumski nivoi HMBG-1 i 3-NT mereni su pomoću enzimsko-imunološke analize.
Rezultati: Nivoi HMBG-1 bili su značajno veći u grupi pacijenata (137,51 pg/mL, p = 0,001), dok nije bilo razlike između dve grupe u serumskim nivoima 3-NT (p = 0,428). Statistički značajna razlika pronađena u nivoima TOS i OSI (p = 0,001 i p = 0,045, respektivno) između pacijenata i kontrola, međutim, nije primećena značajna razlika između grupa u pogledu nivoa TAS (p = 0,228).
Zaključak: Oksidativni stres i nivo HMBG-1 su povećani kod pacijenata sa wAMD-om, a pojačani oksidativni stres može biti povezan sa povećanom nekrozom tkiva i upalom. Stoga bi primenu antioksidativnog tretmana pored rutinske terapije trebalo razmotriti wAMD-u.
Keywords: 3-Nitrotyrosine; HMGB-1; OSI; TAS; TOS; wet type AMD.
2022 Zor Kürşad Ramazan, İsmail Sarı, Biçer Gamze Yıldırım, İnayet Güntürk, Erkut Küçük, Serpil Erşan, Gönül Şeyda Seydel, published by CEON/CEES.