1H, 13C, 15N resonance assignment of the enzyme KdgF from Bacteroides eggerthii

Abstract

To fully utilize carbohydrates from seaweed biomass, the degradation of the family of polysaccharides known as alginates must be understood. A step in the degradation of alginate is the conversion of 4,5-unsaturated monouronates to 4-deoxy-L-erythro-5-hexoseulose catalysed by the enzyme KdgF. In this study BeKdgF from Bacteroides eggerthii from the human gut microbiota has been produced isotopically labelled in Escherichia coli. Here the ¹H, ¹³C, and ¹⁵N NMR chemical shift assignment for BeKdgF is reported.

Keywords: 4-deoxy-L-erythro-5-hexoseulose (DEH); Alginate degradation; Human gut microbiota; KdgF.