Standardized hypoxia-reoxygenation protocol to assess posthypoxic neurobehavioral impairments and molecular mechanisms in Drosophila melanogaster

Jennifer Jung; Aaron T Fehr; Aaron Voigt; Pardes Habib

doi:10.1016/j.xpro.2022.101634

Standardized hypoxia-reoxygenation protocol to assess posthypoxic neurobehavioral impairments and molecular mechanisms in Drosophila melanogaster

STAR Protoc. 2022 Aug 18;3(3):101634. doi: 10.1016/j.xpro.2022.101634. eCollection 2022 Sep 16.

Authors

Jennifer Jung¹, Aaron T Fehr¹, Aaron Voigt², Pardes Habib³

Affiliations

¹ Department of Neurology, Medical Faculty, RWTH Aachen University, 52074 Aachen, Germany.
² Department of Neurology, Medical Faculty, RWTH Aachen University, 52074 Aachen, Germany; JARA BRAIN Institute Molecular Neuroscience and Neuroimaging, Forschungszentrum Jülich GmbH and RWTH Aachen University, 52074 Aachen, Germany.
³ Department of Neurology, Medical Faculty, RWTH Aachen University, 52074 Aachen, Germany; JARA BRAIN Institute Molecular Neuroscience and Neuroimaging, Forschungszentrum Jülich GmbH and RWTH Aachen University, 52074 Aachen, Germany. Electronic address: phabib@ukaachen.de.

Abstract

Hypoxia plays a pivotal role in the pathogenesis of major causes of mortality such as cerebral ischemia. Here, we present a standardized protocol for the induction of global hypoxia and reoxygenation in Drosophila melanogaster, with details on subsequent analysis of mortality, neurobehavioral impairments, and molecular mechanisms. This protocol emphasizes the importance of controlling and monitoring specific environmental parameters to ensure reproducible results. It also highlights profound differences that can arise from variations in the age and genotype of the flies. For complete details on the use and execution of this protocol, please refer to Habib et al. (2021).

Keywords: Behavior; Metabolism; Model organisms; Neuroscience.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Drosophila melanogaster*
Hypoxia
Oxygen*

Substances

Oxygen