Scale space detector for analyzing spatiotemporal ventricular contractility and nuclear morphogenesis in zebrafish

Tanveer Teranikar; Cameron Villarreal; Nabid Salehin; Toluwani Ijaseun; Jessica Lim; Cynthia Dominguez; Vivian Nguyen; Hung Cao; Cheng-Jen Chuong; Juhyun Lee

doi:10.1016/j.isci.2022.104876

Scale space detector for analyzing spatiotemporal ventricular contractility and nuclear morphogenesis in zebrafish

iScience. 2022 Aug 5;25(9):104876. doi: 10.1016/j.isci.2022.104876. eCollection 2022 Sep 16.

Authors

Affiliations

¹ Joint Department of Bioengineering, UT Arlington/UT Southwestern, Arlington, TX, USA.
² Martin High School/ UT Arlington, Arlington, TX, USA.
³ Department of Electrical Engineering, UC Irvine, Irvine, CA, USA.
⁴ Department of Medical Education, TCU and UNTHSC School of Medicine, Fort Worth, TX 76107, USA.

Abstract

In vivo quantitative assessment of structural and functional biomarkers is essential for characterizing the pathophysiology of congenital disorders. In this regard, fixed tissue analysis has offered revolutionary insights into the underlying cellular architecture. However, histological analysis faces major drawbacks with respect to lack of spatiotemporal sampling and tissue artifacts during sample preparation. This study demonstrates the potential of light sheet fluorescence microscopy (LSFM) as a non-invasive, 4D (3days + time) optical sectioning tool for revealing cardiac mechano-transduction in zebrafish. Furthermore, we have described the utility of a scale and size-invariant feature detector, for analyzing individual morphology of fused cardiomyocyte nuclei and characterizing zebrafish ventricular contractility.

Keywords: Biological sciences research methodologies; Biology experimental methods; Biotechnology; Computer science.