This study was conducted to evaluate the effect of glyceraldehyde-3-phosphate dehydrogenase from Schistosoma japonicum (SjGAPDH) on the growth of schistosomula. Quantitative reverse transcription PCR and immunohistochemical analysis were performed to analyze the mRNA levels and immune localization of SjGAPDH. RNA interference experiments were conducted to further examine the role of SjGAPDH in the schistosomula growth of S. japonicum. The results demonstrated that SjGAPDH mRNA was expressed during all stages of S. japonicum development, with its expression gradually increasing over time. SjGAPDH was mainly distributed on the surface and in some parenchymal cells of S. japonicum. Double-stranded RNA-mediated GAPDH knockdown reduced SjGAPDH expression by approximately 59%. Light microscopic observations revealed that the size, length, width, volume, and area of schistosomula in the SjGAPDH interference group were significantly lower than those in the enhanced green fluorescent protein control group. These findings indicate that SjGAPDH may affect the growth of S. japonicum schistosomula and could be a useful target for treating schistosomiasis.
Keywords: Glyceraldehyde-3-phosphate dehydrogenase; Growth; RNA interference; Schistosoma japonicum; Schistosomula.
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