Novel Fluoride- and Chloride-containing Bioactive Glasses for Use in Air Abrasion

Xiaojing Chen; Minyuan Wang; Catherine Kenny; Xiaohui Chen; Natalia Karpukhina; Robert G Hill

doi:10.1016/j.jdent.2022.104252

Novel Fluoride- and Chloride-containing Bioactive Glasses for Use in Air Abrasion

J Dent. 2022 Oct:125:104252. doi: 10.1016/j.jdent.2022.104252. Epub 2022 Aug 26.

Authors

Xiaojing Chen¹, Minyuan Wang², Catherine Kenny³, Xiaohui Chen⁴, Natalia Karpukhina³, Robert G Hill³

Affiliations

¹ Hunan Key Laboratory of Oral Health Research & Academician Workstation for Oral-maxillofacial and Regenerative Medicine & Xiangya Stomatological Hospital & Xiangya School of Stomatology, Central South University, Changsha, 410008, Hunan, China; Institute of Dentistry, Dental Physical Sciences Unit, Barts & The London School of Medicine and Dentistry, Queen Mary University of London, E1 4NS, UK. Electronic address: xiaojing.chen@csu.edu.cn.
² Hunan Key Laboratory of Oral Health Research & Academician Workstation for Oral-maxillofacial and Regenerative Medicine & Xiangya Stomatological Hospital & Xiangya School of Stomatology, Central South University, Changsha, 410008, Hunan, China.
³ Institute of Dentistry, Dental Physical Sciences Unit, Barts & The London School of Medicine and Dentistry, Queen Mary University of London, E1 4NS, UK.
⁴ Division of Dentistry, School of Medical Sciences, The University of Manchester, Manchester, M13 9PL, UK.

PMID: 36030643
DOI: 10.1016/j.jdent.2022.104252

Abstract

Objectives: To investigate the degradation, fluorapatite formation, biological safety and cutting efficiency on dentine of the mixed fluoride- and chloride-containing bioactive glasses (BGs).

Methods: Two series of mixed fluoride- and chloride-containing glasses (GPFCl and GPF2.3Cl series) were synthesized using a melt-quench method. Glass transition temperature (T_g) and the bioactivity in term of glass degradation and fluorapatite formation were evaluated in Tris buffer solution. The cutting efficiency of the powdered BGs (GPF2.3Cl series) on dentine via air abrasion was investigated using white light profilometry and scanning electron microscope. The cytotoxicity of GPF2.3Cl series on human periodontal ligament stem cells (hPLSCs) and oral fibroblasts (OFB) were examined by MTT.

Results: These BGs are highly degradable and able to form fluorapatite within 3h of immersion. The formation of CaF₂ was also found in the high fluoride-containing BGs. The faster glass degradation was evidenced in the BGs with higher chloride. A significant reduction of T_g from 790°C to 463°C was seen with increasing in calcium halide content. Air abrasion on dentine using the low and intermediate chloride-containing glasses demonstrates clear depressions, while no depression was found using the high chloride-containing glass. Moreover, the studied BGs showed no cytotoxicity to hPLSCs and OFB.

Conclusions: The glasses with mixed fluoride and chloride integrate the benefits from the presence of both, showing rapid glass degradation, fast fluorapatite formation, excellent biocompatibility and controllable hardness to provide a selective cutting efficiency on dentine.

Clinical significance: The developed BGs air abrasive with tunable hardness by varying chloride content can selectively cut different dental tissues. In clinic, a relatively hard BG is of great interest for caries preparation, while a soft glass is attractive for tooth cleaning.

Keywords: Air abrasion; Bioactive glass; Caries preparation; Fluorapatite; Mixed fluoride and chloride; Tooth cleaning.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Air Abrasion, Dental* / methods
Apatites
Calcium
Chlorides
Fluorides*
Glass
Humans
Surface Properties
Tromethamine

Substances

Apatites
Chlorides
Tromethamine
fluorapatite
Fluorides
Calcium