Overexpression of histone deacetylase gene 84KHDA909 from poplar confers enhanced tolerance to drought and salt stresses in Arabidopsis

Abstract

Histone deacetylases (HDACs) are important enzymes participating in histone modification and epigenetic regulation of gene transcription. HDACs play an essential role in plant development and stress responses. To date, the role of HDACs is largely uninvestigated in woody plants. In this study, we identified a RPD3/HDA1-type HDAC, named 84KHDA909, from 84 K poplar (Populus alba × Populus glandulosa). The protein encoded by 84KHDA909 contained an HDAC domain. The 84KHDA909 was responsive to drought, salt, and cold stresses, but displayed different expression patterns. Overexpression of 84KHDA909 improved root growth, and conferred enhanced tolerance to drought and salt stresses in Arabidopsis. The transgenic plants displayed greater fresh weight, higher proline content and lower malondialdehyde (MDA) accumulation than the wild type. In the transgenic plants, transcript levels of several genes related to abscisic acid (ABA) biosynthesis and response were altered upon exposure to drought and salt stresses. Our results suggested that 84KHDA909 positively regulates drought and salt stress tolerance through ABA pathway.

Keywords: Abscisic acid; Drought stress; Histone deacetylase; Poplar; Salt stress.