Mechanism analysis of octapeptide from microalgae, Isochrysis zhanjiangensis for suppressing vascular injury and angiogenesis in human umbilical vein endothelial cell

Liyuan Lin; Yuan-Lin He; Yanfei Tang; Pengzhi Hong; Chunxia Zhou; Shengli Sun; Zhong-Ji Qian

doi:10.1016/j.intimp.2022.109149

Mechanism analysis of octapeptide from microalgae, Isochrysis zhanjiangensis for suppressing vascular injury and angiogenesis in human umbilical vein endothelial cell

Int Immunopharmacol. 2022 Oct:111:109149. doi: 10.1016/j.intimp.2022.109149. Epub 2022 Aug 23.

Authors

Liyuan Lin¹, Yuan-Lin He², Yanfei Tang², Pengzhi Hong², Chunxia Zhou¹, Shengli Sun², Zhong-Ji Qian³

Affiliations

¹ School of Chemistry and Environment, College of Food Science and Technology, Shenzhen Institute of Guangdong Ocean University, Guangdong Ocean University, Zhanjiang 524088, China; Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524025, China.
² School of Chemistry and Environment, College of Food Science and Technology, Shenzhen Institute of Guangdong Ocean University, Guangdong Ocean University, Zhanjiang 524088, China.
³ School of Chemistry and Environment, College of Food Science and Technology, Shenzhen Institute of Guangdong Ocean University, Guangdong Ocean University, Zhanjiang 524088, China; Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524025, China. Electronic address: zjqian78@163.com.

PMID: 36027851
DOI: 10.1016/j.intimp.2022.109149

Abstract

Incorporating microalgae active peptides into functional foods is one of the hottest topics in algae research. Ile-Ile-Ala-Val-Glu-Ala-Gly-Cys (IEC) is a novel octapeptide isolated from the microalgae, Isochrysis Zhanjiangensis that inhibits the vascular injury, angiogenesis and has a protective effect on cardiovascular diseases. In this study, IEC can suppress ROS production and inhibit pro-inflammatory factors through the Nrf2/SOD/HO-1 and NF-κB signaling pathways. Additionally, IEC inhibits angiogenesis by reducing the expression of MMP2 and MMP9 via the PI3K/AKT, NF-κB, and MAPK pathways. Molecular docking also demonstrated that IEC possesses an excellent docking effect with SOD, Bcl-2 and VEGFR-2. In conclusion, this study not only provides a new idea for the prevention of cardiovascular diseases, but also proves the possibility of octapeptide (IEC) in functional food and drugs, and further improves the use value of microalgae (Isochrysis Zhanjiangensis).

Keywords: Angiogenesis; Isochrysis Zhanjiangensis; Mechanism; Octapeptide; Vascular injury.

MeSH terms

Cardiovascular Diseases*
Haptophyta* / metabolism
Human Umbilical Vein Endothelial Cells
Humans
Microalgae* / metabolism
Molecular Docking Simulation
NF-kappa B / metabolism
Phosphatidylinositol 3-Kinases / metabolism
Superoxide Dismutase
Vascular System Injuries*

Substances

NF-kappa B
Superoxide Dismutase