Isolation and Characterization of a Newly Discovered Phage, V-YDF132, for Lysing Vibrio harveyi

Shaozhu Kang; Luhao Zhang; Jiaming Liao; Dongzhuo Zhang; Siting Wu; Xin Zhang; Qiwei Qin; Jingguang Wei

doi:10.3390/v14081802

Isolation and Characterization of a Newly Discovered Phage, V-YDF132, for Lysing Vibrio harveyi

Viruses. 2022 Aug 17;14(8):1802. doi: 10.3390/v14081802.

Authors

Shaozhu Kang¹, Luhao Zhang¹, Jiaming Liao¹, Dongzhuo Zhang², Siting Wu¹, Xin Zhang¹, Qiwei Qin^{1

3

4}, Jingguang Wei^{1

5}

Affiliations

¹ College of Marine Sciences, South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China.
² Guangdong Winsun Biological Pharmaceutical Co., Ltd., Guangzhou 511356, China.
³ Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266000, China.
⁴ Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 528478, China.
⁵ Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore.

Abstract

A newly discovered lytic bacteriophage, V-YDF132, which efficiently infects the pathogenic strain of Vibrio harveyi, was isolated from aquaculture water collected in Yangjiang, China. Electron microscopy studies revealed that V-YDF132 belonged to the Siphoviridae family, with an icosahedral head and a long noncontractile tail. The phage has a latent period of 25 min and a burst size of 298 pfu/infected bacterium. V-YDF132 was stable from 37 to 50 °C. It has a wide range of stability (pH 5-11) and can resist adverse external environments. In addition, in vitro the phage V-YDF132 has a strong lytic effect on the host. Genome sequencing results revealed that V-YDF132 has a DNA genome of 84,375 bp with a GC content of 46.97%. In total, 115 putative open reading frames (ORFs) were predicted in the phage V-YDF132 genome. Meanwhile, the phage genome does not contain any known bacterial virulence genes or antimicrobial resistance genes. Comparison of the genomic features of the phage V-YDF132 and phylogenetic analysis revealed that V-YDF132 is a newly discovered Vibrio phage. Multiple genome comparisons and comparative genomics showed that V-YDF132 is in the same genus as Vibrio phages vB_VpS_PG28 (MT735630.2) and VH2_2019 (MN794238.1). Overall, the results indicate that V-YDF132 is potentially applicable for biological control of vibriosis.

Keywords: V-YDF132; Vibrio harveyi; Vibrio phage; aquaculture; lytic bacteriophage.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteriophages* / genetics
Genome, Viral
Myoviridae / genetics
Phylogeny
Siphoviridae*
Vibrio* / genetics

Supplementary concepts

Vibrio harveyi

Grants and funding

This research was funded by the Laboratory of Lingnan Modern Agriculture Project (NT2021008), the Key-Area Research and Development Program of Guangdong Province (2021B0202040002), the earmarked fund for CARS-47-G16, National Key R&D Program of China (2018YFD0900500 and 2018YFC0311300), the National Natural Science Foundation of China (31772882), the Innovation Group Project of Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) (311021006), and the Agricultural Science and Technology Cooperation Project of Institute of New Rural Development of South China Agricultural University (2021XNYNYKJHZGJ042).