Endometrial Cancer in Aspect of Forkhead Box Protein Contribution

Olga Adamczyk-Gruszka; Agata Horecka-Lewitowicz; Jakub Gruszka; Monika Wawszczak-Kasza; Agnieszka Strzelecka; Piotr Lewitowicz

doi:10.3390/ijerph191610403

Endometrial Cancer in Aspect of Forkhead Box Protein Contribution

Int J Environ Res Public Health. 2022 Aug 21;19(16):10403. doi: 10.3390/ijerph191610403.

Authors

Olga Adamczyk-Gruszka^{1

2}, Agata Horecka-Lewitowicz³, Jakub Gruszka⁴, Monika Wawszczak-Kasza⁵, Agnieszka Strzelecka³, Piotr Lewitowicz⁶

Affiliations

¹ Department of Gynaecology and Obstetrics, Collegium Medicum, Jan Kochanowski University, 25-369 Kielce, Poland.
² Department of Obstetrics and Gynaecology, Province Hospital, 25-369 Kielce, Poland.
³ Institute of Health Sciences, Jan Kochanowski University, 25-369 Kielce, Poland.
⁴ 2nd Department of Obstetrics and Gynaecology, Medical University of Warsaw, 02-091 Warsaw, Poland.
⁵ Department of Surgical Medicine with the Laboratory of Medical Genetics, Institute of Medical Sciences, Jan Kochanowski University, 25-369 Kielce, Poland.
⁶ Department of Clinical and Experimental Pathology, Institute of Medical Sciences, Jan Kochanowski University, 25-369 Kielce, Poland.

Abstract

(1) Background: The present study aimed to investigate the influence of forkhead box (FOX) on endometrial cancer (EC) progression. For a better understanding, the driving mechanisms are vital to identifying correlations between genes and their regulators. (2) Methods: The study enrolled one hundred and three white female patients with confirmed EC. For the analysis, we used next-generation sequencing with the Hot Spot Cancer Panel provided by Illumina Inc., San Diego, CA, USA, and an immunohistochemical analysis of FOXA1, FOXP1, and estrogen receptors. (3) Results: FOXA1 silencing led to a worse outcome based on the correlation with FOXA1 (test log-rank p = 0.04220 and HR 2.66, p = 0.033). Moreover, FOX proteins were closely correlated with TP53 and KRAS mutation. (4) Conclusions: Our study confirmed previous reports about FOX box protein in the regulation of tumor growth. A remarkable observation about the unclear crosstalk with crucial genes, as TP53 and KRAS need deeper investigation.

Keywords: Illumina sequencing; endometrial cancer; immunohistochemistry; microsatellite instability.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
Endometrial Neoplasms* / genetics
Endometrial Neoplasms* / pathology
Female
Forkhead Transcription Factors* / genetics
Forkhead Transcription Factors* / metabolism
Humans
Proto-Oncogene Proteins p21(ras) / genetics
Proto-Oncogene Proteins p21(ras) / metabolism
Receptors, Estrogen / metabolism
Repressor Proteins / metabolism

Substances

FOXP1 protein, human
Forkhead Transcription Factors
Receptors, Estrogen
Repressor Proteins
Proto-Oncogene Proteins p21(ras)

Grants and funding

The authors declared that this report received funding from the Polish Government, Minister of Science and Education Office, project no. 024/RID/2018/19 ‘Regional Initiative of Excellence’. Grant value 11, 999, 000. 00 PLN.