Genome-Wide Analysis of the Type-B Authentic Response Regulator Gene Family in Brassica napus

Jin-Jin Jiang; Na Li; Wu-Jun Chen; Yue Wang; Hao Rong; Tao Xie; You-Ping Wang

doi:10.3390/genes13081449

Genome-Wide Analysis of the Type-B Authentic Response Regulator Gene Family in Brassica napus

Genes (Basel). 2022 Aug 15;13(8):1449. doi: 10.3390/genes13081449.

Authors

Jin-Jin Jiang¹, Na Li¹, Wu-Jun Chen¹, Yue Wang², Hao Rong³, Tao Xie¹, You-Ping Wang^{1

4}

Affiliations

¹ Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, China.
² Center for Plant Diversity and Systematics, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China.
³ School of Biological and Food Engineering, Suzhou University, Suzhou 234000, China.
⁴ Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, China.

Abstract

The type-B authentic response regulators (type-B ARRs) are positive regulators of cytokinin signaling and involved in plant growth and stress responses. In this study, we used bioinformatics, RNA-seq, and qPCR to study the phylogenetic and expression pattern of 35 type-B ARRs in Brassica napus. The BnARRs experienced gene expansion and loss during genome polyploidization and were classified into seven groups. Whole-genome duplication (WGD) and segmental duplication were the main forces driving type-B ARR expansion in B. napus. Several BnARRs with specific expression patterns during rapeseed development were identified, including BnARR12/14/18/23/33. Moreover, we found the type-B BnARRs were involved in rapeseed development and stress responses, through participating in cytokinin and ABA signaling pathways. This study revealed the origin, evolutionary history, and expression pattern of type-B ARRs in B. napus and will be helpful to the functional characterization of BnARRs.

Keywords: Brassica napus; authentic response regulators; comparative studies; cytokinin; gene expression pattern.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Brassica napus* / genetics
Brassica rapa* / genetics
Cytokinins
Gene Duplication
Genes, Plant
Genes, Regulator
Genome, Plant / genetics
Phylogeny

Substances

Cytokinins

Grants and funding

This study was funded by the National Natural Science Foundations (31972963), the National Key Research and Development Program of China (2018YFE0108000), the Natural Science Foundation of Jiangsu Province (BK20200292), the Top Talent Support Program of Yangzhou University and the Jiangsu Qinglan Project, the Graduate Training Program for Innovation and Entrepreneurship (SJCX21_1602), the Project of Special Funding for Crop Science Discipline Development (yzuxk202006), and the Priority Academic Program Development of Jiangsu Higher Education Institutions.