Low-scale syringe-made synthesis of 15 N-labeled oligonucleotides

Abstract

Fast and reasonable low-scale (200 nmol) syringe-made synthesis of ¹⁵ N-labeled oligonucleotides representing DNA trinucleotide codons is communicated. All codons were prepared by solid-phase controlled pore glass synthesis column technique via the phosphoramidite method. Twenty-four labeled oligonucleotides covering the DNA genetic code alphabet were prepared using commercially available reagents and affordable equipment in a reasonably short period of time, with acceptable yields and purity for direct applications in mass spectrometry.

Keywords: 15N-labeled oligonucleotides; low-scale; phosphoramidite synthesis; syringe-made codon synthesis.