Occurrence of methylmercury in aerobic environments: Evidence of mercury bacterial methylation based on simulation experiments

Abstract

Methylmercury (MeHg) is mainly produced by anaerobic δ-proteobacteria such as sulfate-reducing bacteria (SRB). However, mercury bio-methylation has also been found to occur in the aerobic soil of the Three Gorges Reservoir (TGR). Using γ-proteobacterial TGR bacteria (TGRB) and δ-proteobacterial Desulfomicrobium escambiense strains, the efficiency of mercury methylation and demethylation was evaluated using an isotope tracer technique. Kinetics simulation showed that the bacterial Hg methylation rate (k_m) of TGRB3 was 4.36 × 10^-9 pg·cell^-1·h^-1, which was significantly lower than that of D. escambiense (170.74 ×10^-9 pg·cell^-1·h^-1) under anaerobic conditions. Under facultative and/or aerobic conditions, D. escambiense could not survive, while the k_m of TGRB3 were 0.35 × 10^-9 and 0.29 × 10^-9 pg·cell^-1·h^-1, respectively. Furthermore, the bacterial MeHg tolerance threshold of TGRB3 was 3.47 × 10^-9 pg·cell^-1, which was 98.6-fold lower than that of D. escambiense under anaerobic conditions. However, the MeHg tolerance threshold of TGRB3 remained at 0.50-0.52 × 10^-9 pg·cell^-1 under facultative and/or aerobic conditions. Notably, bacterial Hg methylation rates (k_m) were higher than the corresponding bacterial MeHg demethylation rates (k_d1). These results establish the contribution of some aerobic and/or facultative anaerobic bacteria to net environmental MeHg production in terrestrial ecosystems and provide a novel understanding of the biogeochemical cycle of MeHg. SYNOPSIS: Hg methylation of facultative and/or aerobic bacteria may contribute to the net production of environmental methylmercury in terrestrial ecosystems.

Keywords: Demethylation; Desulfomicrobium escambiense CGMCC 1.3481; Isotopic tracers; Mercury; Methylation; Oxygen conditions; RaouLtella terrigena TGRB3.