Carbon nanodots combined with loop-mediated isothermal amplification (LAMP) for detection of African swine fever virus (ASFV)

Mikrochim Acta. 2022 Aug 23;189(9):342. doi: 10.1007/s00604-022-05390-7.

Abstract

The spread of African swine fever virus (ASFV) caused huge economic costs, so early detection is particularly important. Here, we established a fluorescence biosensor based on carbon nanodots (CNDs) and loop-mediated isothermal amplification (LAMP) to ultra-sensitively detect ASFV. LAMP with high efficiency produced a large amount of pyro phosphoric acid and caused pH change in a short time. CNDs with strong light stability had a large fluorescence response at the emission wavelength of 585.5 nm to small pH change by the excitation wavelength of 550 nm. The biosensor realized "turn-off-on" mode for ASFV detection with the detection limit as low as 15.21 copies μL-1. In addition, the biosensor had high accuracy in the actual sample assay. Therefore, the biosensor achieved rapid, sensitive, low-cost, and simple detection for ASFV. Moreover, the biosensor broadened the detection pathway of LAMP as a tool with great development prospect.

Keywords: African swine fever virus (ASFV); Carbon nanodot; Fluorescence; Loop-mediated isothermal amplification (LAMP).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • African Swine Fever Virus* / genetics
  • African Swine Fever* / diagnosis
  • Animals
  • Carbon
  • Molecular Diagnostic Techniques
  • Nucleic Acid Amplification Techniques / methods
  • Swine

Substances

  • Carbon

Supplementary concepts

  • LAMP assay