Diagnostic MALDI-TOF MS can differentiate between high and low toxic Staphylococcus aureus bacteraemia isolates as a predictor of patient outcome

Tarcisio Brignoli; Mario Recker; Winnie W Y Lee; Tim Dong; Ranjeet Bhamber; Mahableshwar Albur; Philip Williams; Andrew W Dowsey; Ruth C Massey

doi:10.1099/mic.0.001223

Diagnostic MALDI-TOF MS can differentiate between high and low toxic Staphylococcus aureus bacteraemia isolates as a predictor of patient outcome

Microbiology (Reading). 2022 Aug;168(8):001223. doi: 10.1099/mic.0.001223.

Authors

Tarcisio Brignoli¹, Mario Recker^{2

3}, Winnie W Y Lee¹, Tim Dong⁴, Ranjeet Bhamber⁴, Mahableshwar Albur⁵, Philip Williams⁶, Andrew W Dowsey⁴, Ruth C Massey^{1

7}

Affiliations

¹ School of Cellular and Molecular Medicine, University of Bristol, Bristol, BS8 1TD, UK.
² Centre for Ecology and Conservation, University of Exeter, Penryn, TR10 9FE, UK.
³ Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany.
⁴ Department of Population Health Sciences and Bristol Veterinary School, University of Bristol, Bristol, BS8 2BN, UK.
⁵ Severn Infection Sciences, North Bristol NHS Trust, UK.
⁶ UK Health Security Agency, and University Hospitals Bristol & Weston NHS Trust.
⁷ Schools of Microbiology and Medicine and APC Microbiome Ireland, UCC, Cork, Ireland.

Abstract

Staphylococcus aureus bacteraemia (SAB) is a major cause of blood-stream infection (BSI) in both healthcare and community settings. While the underlying comorbidities of a patient significantly contributes to their susceptibility to and outcome following SAB, recent studies show the importance of the level of cytolytic toxin production by the infecting bacterium. In this study we demonstrate that this cytotoxicity can be determined directly from the diagnostic MALDI-TOF mass spectrum generated in a routine diagnostic laboratory. With further development this information could be used to guide the management and improve the outcomes for SAB patients.

Keywords: bacteraemia; Agr; MALDI-TOF MS diagnosis; Staphylococcus aureus; toxicity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteremia* / diagnosis
Bacteremia* / microbiology
Humans
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Staphylococcal Infections* / diagnosis
Staphylococcal Infections* / microbiology
Staphylococcus aureus

Abstract

Publication types

MeSH terms

Grants and funding