Effects of combination docetaxel with NO treatment to enhance the anti-nasopharyngeal carcinoma efficiency in vitro and in vivo

Lingling Xu; Xidong Wu; Huiqin Liu; Guangyuan Dong; Jiandong Zhan; Guanxue Li; Guanhai Wang; Tao Liu

doi:10.1016/j.ejps.2022.106281

Effects of combination docetaxel with NO treatment to enhance the anti-nasopharyngeal carcinoma efficiency in vitro and in vivo

Eur J Pharm Sci. 2022 Nov 1:178:106281. doi: 10.1016/j.ejps.2022.106281. Epub 2022 Aug 19.

Authors

Lingling Xu¹, Xidong Wu², Huiqin Liu³, Guangyuan Dong³, Jiandong Zhan⁴, Guanxue Li⁵, Guanhai Wang⁶, Tao Liu⁷

Affiliations

¹ Department of Otolaryngology-Head and Neck Surgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, 510080, China; The Second School of Clinical Medicine, Southern Medical University, Guangzhou, 510515, China.
² Department of Drug Safety Evaluation, Jiangxi Testing Center of Medical Instruments, Nanchang, 330029, China.
³ Department of Otolaryngology-Head and Neck Surgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, 510080, China; Shantou University Medical College, Shantou, 515063, China.
⁴ Department of Otolaryngology-Head and Neck Surgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, 510080, China.
⁵ Pediatric Critical Care Medicine, Zhujiang Hospital of Southern Medical University, Guangzhou, 510280, China.
⁶ School of Pharmacy, Guangdong Medical University, Dongguan, 523808, China. Electronic address: wangguanhai@gdmu.edu.cn.
⁷ Department of Otolaryngology-Head and Neck Surgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, 510080, China; The Second School of Clinical Medicine, Southern Medical University, Guangzhou, 510515, China. Electronic address: taoliu18@126.com.

PMID: 35995348
DOI: 10.1016/j.ejps.2022.106281

Abstract

Nasopharyngeal carcinoma (NPC) is one of the major causes of death in Southern China. Due to the insidious location of NPC, the therapeutic effect of locoregionally advanced NPC is still unsatisfactory. In this work, to improve the treatment efficiency, combining DOC and JS-K to inhibit NPC cells (HNE-1) in vitro was investigated, as well as its possible mechanisms. Moreover, the in vivo effects of DOC and JS-K combination treatment were also evaluated in a xenograft model with HNE-1 cells. In vitro experiments including cell proliferation, migration ability, apoptosis, and expression levels of apoptosis-associated proteins revealed that the combination of DOC and JS-K was able to enhance antitumor effects. In vivo results further confirmed a significant treatment effect without obvious toxicity on mice. The present work provides a promising idea for the treatment of locally advanced NPC.

Keywords: Docetaxel; Drug combination; JS-K; Nasopharyngeal carcinoma; Nitric oxide.

MeSH terms

Animals
Apoptosis
Cell Line, Tumor
Cell Proliferation
Docetaxel / pharmacology
Docetaxel / therapeutic use
Humans
Mice
Nasopharyngeal Carcinoma / drug therapy
Nasopharyngeal Neoplasms* / drug therapy
Nasopharyngeal Neoplasms* / metabolism
Nasopharyngeal Neoplasms* / pathology
Xenograft Model Antitumor Assays

Substances

Docetaxel