Effectivity of polyphenolic polysaccharide-proteins﻿ isolated from medicinal plants as potential cellular immune response modulators

Ema Paulovičová; Lucia Paulovičová; Izabela Pawlaczyk-Graja; Roman Gancarz; Mária Kopáčová; Peter Capek

doi:10.1007/s11756-022-01200-w

Effectivity of polyphenolic polysaccharide-proteins isolated from medicinal plants as potential cellular immune response modulators

Biologia (Bratisl). 2022;77(12):3581-3593. doi: 10.1007/s11756-022-01200-w. Epub 2022 Aug 16.

Authors

Ema Paulovičová¹, Lucia Paulovičová¹, Izabela Pawlaczyk-Graja², Roman Gancarz², Mária Kopáčová³, Peter Capek³

Affiliations

¹ Department of Glycoconjugate Immunochemistry, Immunol. & Cell Culture Labs, Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 84538 Bratislava, Slovakia.
² Department of Organic and Pharmaceutical Technology, Faculty of Chemistry, Wrocław University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50370 Wrocław, Poland.
³ Department of Glycomaterials, Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 84538 Bratislava, Slovakia.

Abstract

Traditional medicinal herbs as Echinacea purpurea and Erigeron canadensis are recommended as a complementary supplementation for the treatment of diseases associated with immunological inflammation (e.g. common cold, coughs, bronchitis, upper respiratory infections, immunodeficiencies). This pathologic conditions are accompanied by the wide range of malfunctions or imbalances of the immune system, thus there is increased necessity for search of novel immunomodulation trends and immunopharmacologically active phytosubstances for effective pharmaco-immunomodulatory therapy. Anti-inflammatory immunobiological activity of polyphenolic polysaccharide-proteins of Echinacea purpurea and Erigeron canadensis are still not studied. Our results demonstrated the immunobiological effectivity of selected herbal polyphenolic polysaccharide-proteins isolated from flowers of medicinal plants Echinacea purpurea and Erigeron canadensis resulting into the significant immunostimulation of inflammatory TNF-α, IL-6, IL-1ß and IL-12 cytokines (p < 0.001). Both herbal polyphenolic polysaccharide-proteins triggered cell release of anti-inflammatory interleukin IL-10 (p < 0.001). Furthermore, the inductive cell release of growth factors M-CSF and GM-CSF has been demonstrated (p < 0.001). E. purpurea and E. canadensis polyphenolic polysaccharide-proteins accelerated the efficacy of cellular phagocytosis and free radical release, more pronounced with Erigeron treatment.

Keywords: Cytokines; Echinacea purpurea; Erigeron canadensis; Immunobiological activity; Phagocytosis; Polyphenolic polysaccharide-protein; Proliferation.

© The Author(s), under exclusive licence to Plant Science and Biodiversity Centre, Slovak Academy of Sciences (SAS), Institute of Zoology, Slovak Academy of Sciences (SAS), Institute of Molecular Biology, Slovak Academy of Sciences (SAS) 2022, Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.