Dietary inclusion of anthocyanin-rich black cane silage treated with ferrous sulfate heptahydrate reduces oxidative stress and promotes tender meat production in goats

Rayudika Aprilia Patindra Purba; Ngo Thi Minh Suong; Siwaporn Paengkoum; Jan Thomas Schonewille; Pramote Paengkoum

doi:10.3389/fvets.2022.969321

Dietary inclusion of anthocyanin-rich black cane silage treated with ferrous sulfate heptahydrate reduces oxidative stress and promotes tender meat production in goats

Front Vet Sci. 2022 Aug 4:9:969321. doi: 10.3389/fvets.2022.969321. eCollection 2022.

Authors

Rayudika Aprilia Patindra Purba¹, Ngo Thi Minh Suong^{1

2}, Siwaporn Paengkoum³, Jan Thomas Schonewille⁴, Pramote Paengkoum¹

Affiliations

¹ School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, Thailand.
² School of Animal Sciences, Agriculture Department, Can Tho University, Can Tho, Vietnam.
³ Program in Agriculture, Faculty of Science and Technology, Nakhon Ratchasima Rajabhat University, Nakhon Ratchasima, Thailand.
⁴ Department of Population Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands.

Abstract

Pre-treating anthocyanin-rich black cane with ferrous sulfate heptahydrate (FSH) produces high-quality silage with anthocyanin and nutritional losses. However, it's unclear how to apply this to studies on how FSH-treated silage affects animal performance and meat quality. Therefore, this study aimed to investigate the effects of a standard total mixed ration (TMR) containing anthocyanin-rich black cane silages either with or without dilutions of FSH on animal performance, blood biochemical indices, rumen fermentation, microbial community, and carcass characteristics in meat goats. Forty healthy crossbred Thai-native Anglo-Nubian male goats (14.42 ± 1.4 kg) were used to compare the feasibility of using anthocyanin-rich black cane silage (ABS) as a functional feed resource as opposed to anthocyanin-rich black cane treated with 0.030% commercial FSH silage (ABSF). All goats received a 90-day routine feeding of two isocaloric and isonitrogenous experimental diets: the control group received TMR containing 50% anthocyanin-rich black cane silage (ABS; n = 20), and one group received TMR containing 50% FSH-treating anthocyanin-rich black cane (ABSF; n = 20). As performance indicators, average daily weight gain (ADG) and dry matter intake (DMI) were measured. Samples of meat, blood, and rumen were taken at the end of the experiment. There were no differences in final body weight, ADG, DMI, or ADG/DMI between the two groups. The ABSF group did not differ from the ABS group in terms of rumen pH, but the ABSF had a tendency to lower rumen ammonia N levels, and to higher total volatile fatty acid (VFA) concentrations. Individual VFA concentrations differed, with the ABSF group having more Ruminococcus albus and the ABS group having more methanogenic bacteria. Blood biochemical indices differed, with the ABSF group having lower TBARS concentrations and the ABS group having lower TAC, SOD, CAT, GSH-Px, and GSH-Rx concentrations. In comparison to goat meat from the ABS group, goat meat from the ABSF group contained more intramuscular fat and was more tender. The current results indicate that the feeding of a TMR containing 50% anthocyanin-rich black cane, along with FSH pre-treatment prior to ensiling, reduces oxidative stress and promotes the production of tender meat without affecting animal performance.

Keywords: agricultural waste; anthocyanin; antioxidant capacity; carcass characteristics; fermentation; iron-treated silage; microbial rumen.