Fabrication of silver nanoparticles by a diethylene triamine-hyaluronic acid derivative and use as antibacterial coating

Annalisa Martorana; Giovanna Pitarresi; Fabio Salvatore Palumbo; Valentina Catania; Domenico Schillaci; Nicolò Mauro; Calogero Fiorica; Gaetano Giammona

doi:10.1016/j.carbpol.2022.119861

Fabrication of silver nanoparticles by a diethylene triamine-hyaluronic acid derivative and use as antibacterial coating

Carbohydr Polym. 2022 Nov 1:295:119861. doi: 10.1016/j.carbpol.2022.119861. Epub 2022 Jul 13.

Authors

Annalisa Martorana¹, Giovanna Pitarresi², Fabio Salvatore Palumbo³, Valentina Catania⁴, Domenico Schillaci⁵, Nicolò Mauro⁶, Calogero Fiorica⁷, Gaetano Giammona⁸

Affiliations

¹ Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Via Archirafi 32, 90123 Palermo, Italy. Electronic address: annalisa.martorana@unipa.it.
² Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Via Archirafi 32, 90123 Palermo, Italy. Electronic address: giovanna.pitarresi@unipa.it.
³ Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Via Archirafi 32, 90123 Palermo, Italy. Electronic address: fabiosalvatore.palumbo@unipa.it.
⁴ Department of Earth and Marine Sciences (DiSTeM), University of Palermo, Viale delle Scienze Ed. 16, 90128 Palermo, Italy. Electronic address: valentina.catania@unipa.it.
⁵ Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Via Archirafi 32, 90123 Palermo, Italy. Electronic address: domenico.schillaci@unipa.it.
⁶ Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Via Archirafi 32, 90123 Palermo, Italy. Electronic address: nicolo.mauro@unipa.it.
⁷ Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Via Archirafi 32, 90123 Palermo, Italy. Electronic address: calogero.fiorica@unipa.it.
⁸ Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Via Archirafi 32, 90123 Palermo, Italy. Electronic address: gaetano.giammona@unipa.it.

PMID: 35988982
DOI: 10.1016/j.carbpol.2022.119861

Abstract

In this work a synthetic protocol for the functionalization of hyaluronic acid with diethylenetriamine (DETA) was standardized. HA-DETA derivatives were characterized by NMR and proton carbon correlation analysis HSQC and HMBC to confirm chemical structure. A selected derivative was used to set up a green fabrication procedure for HA-DETA capped silver nanoparticles with the aim to achieve a polymeric based coating with potential application in the treatment of medical devices associated infections. Data from UV-visible spectroscopy, electron scanning and transmission microscope (STEM), photoelectric spectroscopy (XPS) and rheological characterization were combined to characterize the HA-DETA/Ag nanocomposites. HA-DETA stabilized Ag nanoparticles (10-30 nm) were obtained through an UV accelerated production. The viability of MC3T3-E1 was analyzed with the aim of designing a cytocompatible antimicrobial coating. Antibacterial and antibiofilm activity of HA-DETA/Ag nanocomposites have been tested in vitro against Staphylococcus aureus and Pseudomonas aeruginosa both in culture plates than on titanium specimens.

Keywords: Ag nanoparticles; Antibacterial activity; Diethylenetriamine; Hyaluronic acid; Medical device infections.

MeSH terms

Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / pharmacology
DEET
Hyaluronic Acid
Metal Nanoparticles* / chemistry
Polyamines
Silver* / chemistry
Silver* / pharmacology

Substances

Anti-Bacterial Agents
Polyamines
diethylenetriamine
DEET
Silver
Hyaluronic Acid