Long-term microglial phase-specific dynamics during single vessel occlusion and recanalization

Xiaoke Xie; Xuanting Liu; Jiazhu Zhu; Yongxian Xu; Xiaojing Li; Yameng Zheng; Shangyue Gong; Xiao Xiao; Yiwen Chen; Jianmin Zhang; Wei Gong; Ke Si

doi:10.1038/s42003-022-03784-0

Long-term microglial phase-specific dynamics during single vessel occlusion and recanalization

Commun Biol. 2022 Aug 19;5(1):841. doi: 10.1038/s42003-022-03784-0.

Authors

Xiaoke Xie^{1

2}, Xuanting Liu³, Jiazhu Zhu⁴, Yongxian Xu³, Xiaojing Li³, Yameng Zheng³, Shangyue Gong⁵, Xiao Xiao³, Yiwen Chen³, Jianmin Zhang⁵, Wei Gong⁶, Ke Si^{7

8

9

10}

Affiliations

¹ Department of Psychiatry, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China.
² College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, 310027, China.
³ MOE Frontier Science Center for Brain Science & Brain-Machine Integration, NHC and CAMS Key Laboratory of Medical Neurobiology, School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou, 310058, China.
⁴ State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, 310027, China.
⁵ Center for Neuroscience and Department of Neurobiology of the Second Affiliated Hospital, State Key laboratory of Modern Optical Instrumentation, Zhejiang University School of Medicine, Hangzhou, 310009, China.
⁶ MOE Frontier Science Center for Brain Science & Brain-Machine Integration, NHC and CAMS Key Laboratory of Medical Neurobiology, School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou, 310058, China. weigong@zju.edu.cn.
⁷ Department of Psychiatry, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China. kesi@zju.edu.cn.
⁸ College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, 310027, China. kesi@zju.edu.cn.
⁹ MOE Frontier Science Center for Brain Science & Brain-Machine Integration, NHC and CAMS Key Laboratory of Medical Neurobiology, School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou, 310058, China. kesi@zju.edu.cn.
¹⁰ State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, 310027, China. kesi@zju.edu.cn.

Abstract

Vascular occlusion leading to brain dysfunctions is usually considered evoking microglia-induced inflammation response. However, it remains unclear how microglia interact with blood vessels in the development of vascular occlusion-related brain disorders. Here, we illuminate long-term spatiotemporal dynamics of microglia during single vessel occlusion and recanalization. Microglia display remarkable response characteristics in different phases, including acute reaction, rapid diffusion, transition and chronic effect. Fibrinogen-induced microglial cluster promotes major histocompatibility complex II (MHCII) expression. Microglial soma represents a unique filament-shape migration and has slower motility compared to the immediate reaction of processes to occlusion. We capture proliferative microglia redistribute territory. Microglial cluster resolves gradually and microglia recover to resting state both in the morphology and function in the chronic effect phase. Therefore, our study offers a comprehensive analysis of spatiotemporal dynamics of microglia and potential mechanisms to both vessel occlusion and recanalization. Microglial phase-specific response suggests the morphological feature-oriented phased intervention would be an attractive option for vascular occlusion-related diseases treatments.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Brain*
Microglia* / metabolism