Biocompatibility and colorectal anti-cancer activity study of nanosized BaTiO3 coated spinel ferrites

Tahani M Alfareed; Yassine Slimani; Munirah A Almessiere; Muhammad Nawaz; Firdos A Khan; Abdulhadi Baykal; Ebtesam A Al-Suhaimi

doi:10.1038/s41598-022-18306-5

Biocompatibility and colorectal anti-cancer activity study of nanosized BaTiO₃ coated spinel ferrites

Sci Rep. 2022 Aug 19;12(1):14127. doi: 10.1038/s41598-022-18306-5.

Authors

Tahani M Alfareed¹, Yassine Slimani², Munirah A Almessiere^{2

3}, Muhammad Nawaz⁴, Firdos A Khan⁵, Abdulhadi Baykal⁴, Ebtesam A Al-Suhaimi⁶

Affiliations

¹ Master Program of Nanotechnology, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam, 31441, Saudi Arabia.
² Department of Biophysics, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam, 31441, Saudi Arabia.
³ Department of Physics, College of Science, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam, 31441, Saudi Arabia.
⁴ Department of Nanomedicine Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam, 31441, Saudi Arabia.
⁵ Department of Stem Cells, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam, 31441, Saudi Arabia.
⁶ Biology Department, College of Science & Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam, 31441, Saudi Arabia. ealsuhaimi@iau.edu.sa.

Abstract

In the present work, different nanoparticles spinel ferrite series (MFe₂O₄, Co_0.5M_0.5Fe₂O₄; M = Co, Mn, Ni, Mg, Cu, or Zn) have been obtained via sonochemical approach. Then, sol-gel method was employed to design core-shell magnetoelectric nanocomposites by coating these nanoparticles with BaTiO₃ (BTO). The structure and morphology of the prepared samples were examined by X-ray powder diffraction (XRD), scanning electron microscope (SEM) coupled with energy dispersive X-ray spectroscopy (EDX), high-resolution transmission electron microscope (HR-TEM), and zeta potential. XRD analysis showed the presence of spinel ferrite and BTO phases without any trace of a secondary phase. Both phases crystallized in the cubic structure. SEM micrographs illustrated an agglomeration of spherical grains with nonuniformly diphase orientation and different degrees of agglomeration. Moreover, HR-TEM revealed interplanar d-spacing planes that are in good agreement with those of the spinel ferrite phase and BTO phase. These techniques along with EDX analyses confirmed the successful formation of the desired nanocomposites. Zeta potential was also investigated. The biological influence of (MFe₂O₄, CoMFe) MNPs and core-shell (MFe₂O₄@BTO, CoMFe@BTO) magnetoelectric nanocomposites were examined by MTT and DAPI assays. Post 48 h of treatments, the anticancer activity of MNPs and MENCs was investigated on human colorectal carcinoma cells (HCT-116) against the cytocompatibility of normal non-cancerous cells (HEK-293). It was established that MNPs possess anti-colon cancer capability while MENCs exhibited a recovery effect due to the presence of a protective biocompatible BTO layer. RBCs hemolytic effect of NPs has ranged from non- to low-hemolytic effect. This effect that could be attributed to the surface charge from zeta potential, also the CoMnFe possesses the stable and lowest zeta potential in comparison with CoFe₂O₄ and MnFe₂O₄ also to the protective effect of shell. These findings open up wide prospects for biomedical applications of MNPs as anticancer and MENCs as promising drug nanocarriers.

MeSH terms

Aluminum Oxide
Barium Compounds
Colorectal Neoplasms* / drug therapy
Ferric Compounds
HEK293 Cells
Humans
Magnesium Oxide
Titanium

Substances

Barium Compounds
Ferric Compounds
spinell
barium titanate(IV)
ferrite
Magnesium Oxide
Titanium
Aluminum Oxide