Histological and hemodynamic characterization of corpus luteum throughout the luteal phase in pregnant and non-pregnant buffalos in relation to nitric oxide levels based on its anatomical determination

Samer M Daghash; Noha A E Yasin; Elshymaa A Abdelnaby; Ibrahim A Emam; Ayman Tolba; Yara S Abouelela

doi:10.3389/fvets.2022.896581

Histological and hemodynamic characterization of corpus luteum throughout the luteal phase in pregnant and non-pregnant buffalos in relation to nitric oxide levels based on its anatomical determination

Front Vet Sci. 2022 Jul 28:9:896581. doi: 10.3389/fvets.2022.896581. eCollection 2022.

Authors

Samer M Daghash¹, Noha A E Yasin², Elshymaa A Abdelnaby³, Ibrahim A Emam⁴, Ayman Tolba¹, Yara S Abouelela¹

Affiliations

¹ Anatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
² Cytology and Histology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
³ Theriogenology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
⁴ Department of Surgery, Anaesthesiology and Radiology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.

Abstract

This study aims to compare the complete growth and development of corpus luteum (CL) in domestic buffalos from day 5 until day 40 after ovulation either in pregnant or non-pregnant animals and whether luteal vascularity (LV) with progesterone (P4) and nitric oxide (NO) could determine luteal functionality or not. Pluriparous buffalos (Bubalus bubalis) were categorized as pregnant (n = 6) or non-pregnant (n = 9) after pregnancy check at day 25. Animals were subjected to ultrasound analysis to determine the CL area (cm²) and LV. Blood sampling was performed following the Doppler examination. Ovarian tissue samples from non-pregnant buffalo genitalia (n = 18) and early pregnant buffalo genitalia (n = 3) were collected from great abattoirs. Luteal Doppler indices were lower in the pregnant group, while peak systolic velocity (PSV) was increased (p < 0.05) in the same pregnant females. Both P4 and NOMs were elevated (p < 0.05) in the pregnant group. There was a positive correlation (p < 0.01) between P4 and CL PSV. Based on our macroscopical examination, the CL of non-pregnant buffalos was classified into four stages. Histologically, stage I showed that CL was covered by a highly vascularized connective tissue (CT) capsule. It consisted of small and large lutein cells, whereas stage II was similar to stage I except for the presence of numerous fibroblast cells and vacuolated cells. Stage III was characterized by increasing the number of collagen fibers and the thickness of the blood vessels. Stage IV revealed thickening of the CT capsule and septae, regressed capillaries and arterioles, in addition to shrunken degenerated lutein cells. CL of pregnant buffalos revealed the same structure as CL at stage II. CL area was increased in the pregnant group. The collective data suggested that evaluation of the luteal artery could be extremely helpful to determine the potential benefits of colored and pulsed Doppler in CL vascularization assessment in both luteal and early pregnancy phases.

Keywords: Doppler; buffalos; corpus luteum; histology; pregnant.