Phenotypic and Genotypic Diversity of Ascochyta fabae Populations in Southern Australia

Sara N Blake; Robert C Lee; Michelle H Russ; Elizabeth A Farquharson; Jade A Rose; Herdina; Shashi N Goonetilleke; Lina M Farfan-Caceres; Johannes W Debler; Robert A Syme; Jennifer A Davidson

doi:10.3389/fpls.2022.918211

Phenotypic and Genotypic Diversity of Ascochyta fabae Populations in Southern Australia

Front Plant Sci. 2022 Aug 2:13:918211. doi: 10.3389/fpls.2022.918211. eCollection 2022.

Affiliations

¹ Pulse and Oilseed Pathology, Plant Health and Biosecurity, Crop Sciences, South Australian Research and Development Institute, Adelaide, SA, Australia.
² Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia.
³ Crop Improvement, Plant Health and Biosecurity, South Australian Research and Development Institute, Adelaide, SA, Australia.

Abstract

Ascochyta fabae Speg. is a serious foliar fungal disease of faba bean and a constraint to production worldwide. This study investigated the phenotypic and genotypic diversity of the A. fabae pathogen population in southern Australia and the pathogenic variability of the population was examined on a differential set of faba bean cultivars. The host set was inoculated with 154 A. fabae isolates collected from 2015 to 2018 and a range of disease reactions from high to low aggressiveness was observed. Eighty percent of isolates collected from 2015 to 2018 were categorized as pathogenicity group (PG) PG-2 (pathogenic on Farah) and were detected in every region in each year of collection. Four percent of isolates were non-pathogenic on Farah and designated as PG-1. A small group of isolates (16%) were pathogenic on the most resistant differential cultivars, PBA Samira or Nura, and these isolates were designated PG-3. Mating types of 311 isolates collected between 1991 and 2018 were determined and showed an equal ratio of MAT1-1 and MAT1-2 in the southern Australian population. The genetic diversity and population structure of 305 isolates were examined using DArTseq genotyping, and results suggest no association of genotype with any of the population descriptors viz.: collection year, region, host cultivar, mating type, or PG. A Genome-Wide Association Study (GWAS) was performed to assess genetic association with pathogenicity traits and a significant trait-associated genomic locus for disease in Farah AR and PBA Zahra, and PG was revealed. The high frequency of mating of A. fabae indicated by the wide distribution of the two mating types means changes to virulence genes would be quickly distributed to other genotypes. Continued monitoring of the A. fabae pathogen population through pathogenicity testing will be important to identify any increases in aggressiveness or emergence of novel PGs. GWAS and future genetic studies using biparental mating populations could be useful for identifying virulence genes responsible for the observed changes in pathogenicity.

Keywords: DArTseq; Didymella fabae; Vicia faba; ascochyta blight; mating type; pathogenicity; pathogenicity group.