Conformational and thermal properties of gluten in wheat dough as affected by bacterial cellulose

Abstract

Bacterial cellulose (BC), an important category of polysaccharides, was investigated as a texture improver in bakery products. This study focused on the changes in the conformational and thermal properties of gluten in the wheat dough system as affected by BC. Significant reductions in the free-SH content, fluorescence intensity, and surface hydrophobicity index (H₀) were observed as a result of the increased BC addition. The electrophoresis profile (SDS-PAGE) and size exclusion (SE-HPLC) revealed the variation in molecular weight distribution, and the increase in the content of the 40-91 kDa molecular weight was at the expense of a decrease in the amount of the corresponding 10-40 kDa. When 0.1 % BC was added, both the α-helix and β-sheet contents increased as a result of enhanced chemical interactions, thereby contributing to the gluten matrix with higher thermal stability. Further supplementation interfered with the current ordered gluten structure, which could be supported by the lower α-helix/β-sheet content ratio and the decreased degradation temperature (T_d) of gluten with 0.2 % BC. However, the observed decrease in the ratio of β-turns to β-sheets and weight loss at 600 °C indicated that a reconstructed gluten matrix induced by extra BC addition was formed to maintain the structural stability.

Keywords: Bacterial cellulose; Conformational property; Gluten protein; Thermal property.