AbstractObjective: To explore the distribution characteristics and clinical significance of peripheral blood monocyte subgroups in patients with diffuse large Bcell lymphoma(DLBCL).
Methods: The percentage of peripheral blood monocyte subsets of 82 DLBCL patients (including 32 newly diagnosis, 29 remission and 21 relapse) and 30 healthy controls were detected by flow cytometry, and the correlation with the clinical characteristics and its diagnostic value of DLBCL were analyzed.
Results: The proportion of intermediate monocytes in patients with newly diagnosed DLBCL group was higher than that in healthy controls (t=5.888, P<0.01). The proportion in relapsed group was higher than those in newly diagnosed DLBCL group（t=2.106，P=0.04） and remission group （t=6.882， P＜0.01）, and the proportion of intermediate monocytes in newly diagnosed DLBCL group was higher than that in Remission group (t=3.969, P<0.01). With the increase of International Prognostic Index (IPI) score, the percentage of intermediate monocytes in patients with DLBCL increased (r=0.37). Furthermore, when the proportion of intermediate monocytes was 10.91% as the cutoff value, the sensitivity and specificity of the whole sample were 90.60% and 9100%, respectively.
Conclusion: The disease progression is related to the increased intermediate monocytes, which can be used as a potential diagnostic index for DLBCL.
题目: 外周血中间型单核细胞增多与弥漫大B细胞淋巴瘤疾病进展的相关性研究.
目的: 探讨弥漫大B细胞淋巴瘤（DLBCL）患者外周血单核细胞亚群变化及临床意义.
方法: 采用流式细胞术检测82例DLBCL患者(包括32例初诊、 29例缓解以及21例复发)和30例健康对照者外周血单核细胞亚群百分比，并分析其与DLBCL患者临床特征的相关性及其诊断价值.
结果: 与健康对照组相比，DLBCL初诊组患者中间型单核细胞亚群占总单核细胞比例明显升高（t=5.888，P＜0.01）。DLBCL复发组患者中间型单核细胞亚群所占比例明显高于初诊组（t=2.106，P=0.04）和缓解组（t=6.882，P＜0.01），并且初诊组患者中间型单核细胞亚群比例高于缓解组（t=3.969，P＜0.01）。随着国际预后指数（IPI）评分的升高，DLBCL患者的中间型单核细胞亚型百分比增多（r=0.37）。当截点值为中间型单核细胞比例10.91%时，整体样本作为早期诊断指标的灵敏度和特异度分别为90.60%和91.00.
结论: DLBCL患者疾病进展与中间型单核细胞增多相关，可作为DLBCL潜在的诊断指标.
Keywords: diagnostic index; diffuse large Bcell lymphoma; disease progression; flow cytometry; intermediate monocytes.