Preparation of Drosophila Tissues and Organs for Transmission Electron Microscopy

Olympia-Ekaterini Psathaki; Achim Paululat

doi:10.1007/978-1-0716-2541-5_19

Preparation of Drosophila Tissues and Organs for Transmission Electron Microscopy

Methods Mol Biol. 2022:2540:361-385. doi: 10.1007/978-1-0716-2541-5_19.

Authors

Olympia-Ekaterini Psathaki^{1

2}, Achim Paululat³

Affiliations

¹ University of Osnabrück, Department of Zoology and Developmental Biology, Osnabrück, Germany.
² University of Osnabrück, Center of Cellular Nanoanalytics, Integrated Bioimaging Facility Osnabrück (iBiOs), Osnabrück, Germany.
³ University of Osnabrück, Department of Zoology and Developmental Biology, Osnabrück, Germany. achim.paululat@uos.de.

PMID: 35980589
DOI: 10.1007/978-1-0716-2541-5_19

Abstract

Transmission electron microscopy (TEM) is the method of choice to image the ultrastructure of cells or tissues. TEM allows the visualization of molecular complexes up to an atomic resolution. Thus, TEM data have led to important conclusions about cellular processes and supported findings obtained by functional analyses. In this chapter, we describe the preparation of Drosophila tissues for TEM and provide reliable step-by-step protocols for applying classical chemical fixation or high-pressure freezing-freeze substitution (HPF-FS) to preserve cellular structures.

Keywords: Chemical fixation; Drosophila; Freeze-substitution; High-pressure freezing; Transmission electron microscopy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cryopreservation* / methods
Drosophila*
Freeze Substitution / methods
Histological Techniques
Microscopy, Electron, Transmission